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Literature summary for 1.1.1.215 extracted from

  • Kuivanen, J.; Arvas, M.; Richard, P.
    Clustered genes encoding 2-keto-L-gulonate reductase and L-idonate 5-dehydrogenase in the novel fungal D-glucuronic acid pathway (2017), Front. Microbiol., 8, 225 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.215

Cloned(Commentary)

Cloned (Comment) Organism
gene gluD, clustered with gene gluE, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strains ATCC 90845 and modified CEN.PK2, subcloning in Escherichia coli strain TOP10 Aspergillus niger

Protein Variants

Protein Variants Comment Organism
additional information identification of a gene cluster encoding NADPH-dependent, L-idonate forming, 2-keto-L-gulonate reductase and NAD+-dependent L-idonate 5-dehydrogenase which forms 5-keto-D-gluconate. These genes are involved in the fungal D-glcUA catabolism and the reaction catalyzed by the latter enzyme is a direct continuation for the previously identified reaction by the action of GluC. Generation of a gene gluD deletion mutant strain, phenotype, overview. Deletion of gluD does not result in reduced or no growth on D-glcUA as sole carbon source, but it results in a phenotype of accumulating 2-keto-L-gulonate when cultivating on D-glcUA Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.6
-
 L-Idonate pH 8, temperature not specified in the publication Aspergillus niger
25.3
-
 2-oxo-L-gulonate pH 7, temperature not specified in the publication Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A0A1D8RK43
-
-
Aspergillus niger ATCC 1015 A0A1D8RK43
-
-
Aspergillus niger CBS 113.46 A0A1D8RK43
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-L-gulonate + NADPH + H+
-
 Aspergillus niger L-idonate + NADP+
-
 r
2-oxo-L-gulonate + NADPH + H+
-
 Aspergillus niger ATCC 1015 L-idonate + NADP+
-
 r
2-oxo-L-gulonate + NADPH + H+
-
 Aspergillus niger CBS 113.46 L-idonate + NADP+
-
 r
L-idonate + NADP+
-
 Aspergillus niger 2-oxo-L-gulonate + NADPH + H+
-
 r
L-idonate + NADP+
-
 Aspergillus niger ATCC 1015 2-oxo-L-gulonate + NADPH + H+
-
 r
L-idonate + NADP+
-
 Aspergillus niger CBS 113.46 2-oxo-L-gulonate + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
2-keto-L-gulonate reductase
-
 Aspergillus niger
GluD
-
 Aspergillus niger
NADPH requiring 2-keto-L-gulonate reductase
-
 Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 L-Idonate pH 8, temperature not specified in the publication Aspergillus niger
21.4
-
 2-oxo-L-gulonate pH 7, temperature not specified in the publication Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, L-idonate oxidation Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADP+ strict requirement for NADP+/NADPH as cofactors Aspergillus niger
NADPH strict requirement for NADP+/NADPH as cofactors Aspergillus niger

General Information

General Information Comment Organism
malfunction gluD deletion results in accumulation of 2-keto-L-gulonate in the liquid cultivation, while the gluE deletion results in reduced growth and cessation of the D-glucuronic acid catabolism Aspergillus niger
metabolism in the filamentous fungus Aspergillus niger, the enzymes that are known to be part of the D-glucuronic acid catabolism pathway are the NADPH requiring D-glucuronic acid reductase forming L-gulonate and the NADH requiring 2-keto-L-gulonate reductase that forms L-idonate. With the aid of RNA sequencing two more enzymes of the pathway are identified. The first is a NADPH requiring 2-keto-L-gulonate reductase that forms L-idonate, GluD. The second is a NAD+ requiring L-idonate 5-dehydrogenase forming 5-keto-gluconate, GluE (EC 1.1.1.366). The genes coding for these two enzymes are clustered and share the same bidirectional promoter Aspergillus niger
physiological function a GluD deletion mutant does not show reduced growth when cultivated on agar plate with D-glucuronate as sole carbon source. In the liquid cultivation on D-glucuronate, 2-oxo-L-gulonate accumulates in the medium after D-glucuronate is consumed Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.087
-
 L-Idonate pH 7.0, temperature not specified in the publication Aspergillus niger
0.087
-
 L-Idonate pH 8, temperature not specified in the publication Aspergillus niger
0.846
-
 2-oxo-L-gulonate pH 7, temperature not specified in the publication Aspergillus niger
0.85
-
 2-dehydro-D-gluconate pH 8.0, temperature not specified in the publication Aspergillus niger