Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.218 extracted from

  • Yamano, S.; Nishida, F; Toki, S.
    Guinea-pig liver morphine 6-dehydrogenase as a naloxone reductase (1986), Biochem. Pharmacol., 35, 4321-4326.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CuSO4
-
Cavia porcellus
Ketamine
-
Cavia porcellus
lithocholic acid
-
Cavia porcellus
additional information no or weak inhibition by pyrazole, barbital Cavia porcellus
Phenylarsine oxide
-
Cavia porcellus
prostaglandin E1
-
Cavia porcellus
quercitrin
-
Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
NADH
-
Cavia porcellus
0.0065
-
NADPH
-
Cavia porcellus
0.27
-
naloxone cosubstrate NADPH Cavia porcellus
0.44
-
naloxone cosubstrate NADH Cavia porcellus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cavia porcellus 5829
-

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Cavia porcellus Hartley
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, various chromatographic techniques Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.86
-
morphine, NADP+ Cavia porcellus
0.9
-
naloxone, NADPH Cavia porcellus

Storage Stability

Storage Stability Organism
2-4┬░C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week Cavia porcellus
4┬░C, 80% of the activity after a week in 50 mM sodium phosphate buffer, pH 6.0, 100 mM 2-mercaptoethanol, more stable at pH 6.0 than at pH 8.0 Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
morphine + NADP+
-
Cavia porcellus morphinone + NADPH + H+
-
?
morphine + NADP+
-
Cavia porcellus Hartley morphinone + NADPH + H+
-
?
naloxone + NAD(P)H
-
Cavia porcellus 6alpha-naloxol + NADP+ stereospecific reduction ?
naloxone + NAD(P)H
-
Cavia porcellus Hartley 6alpha-naloxol + NADP+ stereospecific reduction ?
naloxone + NADH + H+
-
Cavia porcellus ?
-
r
naloxone + NADH + H+
-
Cavia porcellus Hartley ?
-
r

Synonyms

Synonyms Comment Organism
naloxone reductase
-
Cavia porcellus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
enzyme assay, reduction of naloxone Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
with NADH als cofactor, 67 mM sodium phosphate buffer Cavia porcellus
6.8
-
with NADPH as cofactor, 67 mM sodium phosphate buffer Cavia porcellus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
at pH6.0 more stable than at pH 8.0 on storage at 4┬░C Cavia porcellus

Cofactor

Cofactor Comment Organism Structure
NAD(P)+ utilized both with nearly equal efficiency Cavia porcellus
NAD(P)H NADPH: 2.7 times as effective as NADH Cavia porcellus