Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ketamine | - |
Mesocricetus auratus | |
lithocholic acid | moderate inhibitory effect | Mesocricetus auratus | |
additional information | competitive inhibitors: testosterone, 5alpha-androstan-17beta-ol-3-one, 5beta-androsteran-17beta-ol-3-one; no inhibition by indomethacine; no or weak inhibition by pyrazole, barbital | Mesocricetus auratus | |
NEM | - |
Mesocricetus auratus | |
p-chloromercuribenzoic acid | - |
Mesocricetus auratus | |
Phenylarsine oxide | - |
Mesocricetus auratus | |
quercetin | weak inhibitory effect | Mesocricetus auratus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
5alpha-androstan-17beta-ol-3-one | similar values of other 17beta-hydroxysteroids | Mesocricetus auratus | |
0.009 | - |
testosterone | cosubstrate NAD+ | Mesocricetus auratus | |
1.25 | - |
morphine | cosubstrate NAD+ | Mesocricetus auratus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mesocricetus auratus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
native enzyme, gel filtration | Mesocricetus auratus |
38000 | - |
denatured enzyme, SDS-PAGE | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
Syrian hamster | - |
Purification (Comment) | Organism |
---|---|
gel filtration, various chromatographic techniques | Mesocricetus auratus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mesocricetus auratus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
morphine, NAD+ | Mesocricetus auratus |
Storage Stability | Organism |
---|---|
-80°C, 20% glycerol, v/v, stable at least four months | Mesocricetus auratus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + NAD(P)+ | only weak enzyme activity for codeine | Mesocricetus auratus | codeinone + NAD(P)H | - |
? | |
morphine + NADP+ | enzyme activity only with NAD+ | Mesocricetus auratus | morphinone + NADPH + H+ | - |
? | |
normorphine + NAD(P)+ | best substrate, oxidation at 139% the rate of morphine oxidation | Mesocricetus auratus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Mesocricetus auratus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay | Mesocricetus auratus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
in glycine-NaCl-NaOH buffer | Mesocricetus auratus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | with NADP+ enzyme activity less than 5% those found with NAD+ | Mesocricetus auratus |