Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Camellia sinensis |
into expression vector pQE-30 UA, and transformed into Escherichia coli M15 pREP-4 competent cells | Camellia sinensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
abscisic acid | decreases expression | Camellia sinensis | |
additional information | is unaffected by gibberellic acid treatment | Camellia sinensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Camellia sinensis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38670 | - |
sequence analysis | Camellia sinensis |
38670 | - |
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide | Camellia sinensis |
41000 | - |
x * 41000, SDS-PAGE | Camellia sinensis |
41000 | - |
x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide | Camellia sinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | Q6DT40 | - |
- |
Camellia sinensis | Q9S787 | - |
- |
Camellia sinensis (L.) O. Kuntze | Q6DT40 | - |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA column | Camellia sinensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | expression is maximum in younger rather than older leaves | Camellia sinensis | - |
leaf | expression is maximal in younger rather than older leaves | Camellia sinensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42.8 | - |
pH 7.0, 25°C | Camellia sinensis |
42850 | - |
recombinant protein | Camellia sinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-dihydroquercetin + NADPH + H+ | - |
Camellia sinensis | cis-3,4-leucocyanidin + NADP+ | - |
? | |
(+)-dihydroquercetin + NADPH + H+ | - |
Camellia sinensis (L.) O. Kuntze | cis-3,4-leucocyanidin + NADP+ | - |
? | |
dihydroquercetin + NADPH + H+ | - |
Camellia sinensis | leucocyanidin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE | Camellia sinensis |
? | x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide | Camellia sinensis |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Camellia sinensis |
dihydroflavonol 4-reductase | - |
Camellia sinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Camellia sinensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camellia sinensis | calculated | - |
6.2 |
Camellia sinensis | sequence analysis | - |
6.22 |
Organism | Comment | Expression |
---|---|---|
Camellia sinensis | in response to drought stress | down |
Camellia sinensis | expression is down-regulated in response to drought stress and abscisic acid, and unaffected by gibberellic acid treatment | down |
Camellia sinensis | in response to wounding, with concomitant modulation of catechins content. Expression is maximum in younger rather than older leaves | up |
Camellia sinensis | expression is up-regulated in response to wounding, with concomitant modulation of catechins content | up |