Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Komagataella phaffii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fusion of ADH to the N-terminal His-tag causes a strong improvement of about 95% in activity | Komagataella phaffii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0161 | - |
NADPH | pH 7.0, 25°C | Komagataella phaffii | |
1.15 | - |
(4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one | pH 7.0, 25°C | Komagataella phaffii | |
5.2 | - |
benzaldehyde | pH 7.0, 25°C | Komagataella phaffii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
- |
Komagataella phaffii |
41400 | - |
- |
Komagataella phaffii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella phaffii | C4R4L0 | - |
- |
Komagataella phaffii GS115 | C4R4L0 | - |
- |
Storage Stability | Organism |
---|---|
4°C, 50% loss of activity in 100 days | Komagataella phaffii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-hex-2-enal + NADPH + H+ | 97% of the activity with benzaldehyde | Komagataella phaffii | (2E)-hex-2-en-1-ol + NADP+ | - |
? | |
(2E)-hex-2-enal + NADPH + H+ | 97% of the activity with benzaldehyde | Komagataella phaffii GS115 | (2E)-hex-2-en-1-ol + NADP+ | - |
? | |
(2S)-hexan-2-ol + NADP+ | - |
Komagataella phaffii | hexan-2-one + NADPH + H+ | - |
r | |
(2S)-hexan-2-ol + NADP+ | - |
Komagataella phaffii GS115 | hexan-2-one + NADPH + H+ | - |
r | |
(4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one + NADPH + H+ | enzyme specifically reduces the S-enantiomer | Komagataella phaffii | (2S)-1,1,1-trifluoro-2-phenylpentane-2,4-diol + NADP+ | - |
? | |
2-methylpentanal + NADPH + H+ | 249% of the activity with benzaldehyde | Komagataella phaffii | 2-methyl-pentan-1-ol + NADP+ | - |
? | |
benzaldehyde + NADPH + H+ | - |
Komagataella phaffii | benzyl alcohol + NADP+ | - |
? | |
benzaldehyde + NADPH + H+ | - |
Komagataella phaffii GS115 | benzyl alcohol + NADP+ | - |
? | |
butanal + NADPH + H+ | 176% of the activity with benzaldehyde | Komagataella phaffii | butan-1-ol + NADP+ | - |
? | |
butanal + NADPH + H+ | 176% of the activity with benzaldehyde | Komagataella phaffii GS115 | butan-1-ol + NADP+ | - |
? | |
heptan-2-one + NADPH + H+ | - |
Komagataella phaffii | (2S)-heptan-2-ol + NADP+ | 99% conversion, 99% enantiomeric excess | r | |
hexan-2-one + NADPH + H+ | - |
Komagataella phaffii | (2S)-hexan-2-ol + NADP+ | 99% conversion, 99% enantiomeric excess | r | |
hexanal + NADPH + H+ | 74% of the activity with benzaldehyde | Komagataella phaffii | hexan-1-ol + NADP+ | - |
? | |
additional information | linear and branched aliphatic aldehydes are good substrates | Komagataella phaffii | ? | - |
? | |
additional information | linear and branched aliphatic aldehydes are good substrates | Komagataella phaffii GS115 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, SDS-PAGE, x * 41400, calculated | Komagataella phaffii |
Synonyms | Comment | Organism |
---|---|---|
PAS_chr3_0449 | - |
Komagataella phaffii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Komagataella phaffii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, rapid inactivation | Komagataella phaffii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.5 | - |
(4S)-5,5,5-trifluoro-4-hydroxy-4-phenylpentan-2-one | pH 7.0, 25°C | Komagataella phaffii | |
54.4 | - |
benzaldehyde | pH 7.0, 25°C | Komagataella phaffii | |
58.4 | - |
NADPH | pH 7.0, 25°C | Komagataella phaffii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Komagataella phaffii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Komagataella phaffii |