Protein Variants | Comment | Organism |
---|---|---|
C260A | no oxidation of UDP-glucose to glucuronic acid, but capable of both reducing the aldehyde intermediate and oxidizing the hydrated form of the aldehyde intermediate, protein is expressed in inclusion bodies | Streptococcus pyogenes |
E141Q | kcat-value 10fold lower than wild-type | Streptococcus pyogenes |
E145Q | kcat-value 10fold lower than wild-type | Streptococcus pyogenes |
T118A | 160fold reduction of kcat value | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
UDP-glucose | wild-type | Streptococcus pyogenes | |
0.058 | - |
aldehyde intermediate | - |
Streptococcus pyogenes | |
0.059 | - |
UDP-glucose | mutant T118A | Streptococcus pyogenes | |
0.06 | - |
UDP-glucose | mutant E141Q | Streptococcus pyogenes | |
0.065 | - |
NAD+ | wild-type | Streptococcus pyogenes | |
0.125 | - |
UDP-glucose | mutant E145Q | Streptococcus pyogenes | |
0.135 | - |
NAD+ | mutant E141Q | Streptococcus pyogenes | |
0.187 | - |
NAD+ | mutant E145Q | Streptococcus pyogenes | |
0.4 | - |
NAD+ | mutant T118A | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + 2 NAD+ + H2O = UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | mechanism, ordered water molecule H-bonded to T118 serves as catalytic base in hydride transfer | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Streptococcus pyogenes | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
aldehyde intermediate | - |
Streptococcus pyogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptococcus pyogenes |