Cloned (Comment) | Organism |
---|---|
hUGDH gene is cloned from a LNCaP cDNA library, from LNCaP C33 and C81 cells, expression of His6-tagged UGDH in Escherichia coli, expression of UGDH mutant D280N in HEK.293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D280N | an inactive UGDH mutant | Homo sapiens |
additional information | UGDH overexpression stimulates hyaluronan production in HEK293 cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | Homo sapiens | - |
UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged UGDH from Escherichia coli by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | high expression level | Homo sapiens | - |
LNCaP cell | - |
Homo sapiens | - |
PC-3M-LN4 cell | prostate tumor cell line derived from PC-3 cells | Homo sapiens | - |
prostate | high expression level | Homo sapiens | - |
prostate cancer cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-glucuronic acid + 2 NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose dehydrogenase | - |
Homo sapiens |
UGDH | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | dihydrotestosterone increases UGDH expression 2.5fold in androgen-dependent cells. However, upregulation of UGDH does not affect hyaluronan synthase expression or enhance hyaluronan production | up |
General Information | Comment | Organism |
---|---|---|
additional information | dysregulated expression of UGDH can promote the development of androgen independent tumor cell growth by increasing available levels of intracellular androgen. UGDH activity is the rate limiting factor in solubilization of excess androgen from prostate tumor cells, overview | Homo sapiens |
physiological function | UGDH oxidizes UDP-glucose to UDP-glucuronate, an essential precursor for production of hyaluronan, proteoglycans, and xenobiotic glucuronides. High levels of hyaluronan turnover in prostate cancer are correlated with aggressive progression. UGDH expression is high in the normal prostate even though hyaluronan accumulation is virtually undetectable. The enzyme's common role in the prostate may be to provide precursors for glucuronosyltransferase enzymes, which inactivate and solubilize androgens by glucuronidation. Androgen dependence of UGDH, glucuronosyltransferase, and hyaluronan synthase expression, overview | Homo sapiens |