Cloned (Comment) | Organism |
---|---|
gene PK-ISPD1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F' | Perilla frutescens var. hirtella |
gene PT-ISPD2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F' | Perilla frutescens |
gene PT-ISPD2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F' | Perilla frutescens var. hirtella |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella | - |
(-)-isopiperitenone + NADH + H+ | - |
r | |
(-)-cis-isopiperitenol + NAD+ | Perilla frutescens | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | Perilla frutescens 5598 | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella 5712 | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella 5712 | - |
(-)-isopiperitenone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Perilla frutescens | W5VJJ6 | PT-type | - |
Perilla frutescens 5598 | W5VJJ6 | PT-type | - |
Perilla frutescens var. hirtella | W5VJM4 | - |
- |
Perilla frutescens var. hirtella | W5VJT8 | PK-type, not found in not found in PT-type strains nos. 5598 and 5601 | - |
Perilla frutescens var. hirtella 5712 | W5VJM4 | - |
- |
Perilla frutescens var. hirtella 5712 | W5VJT8 | PK-type, not found in not found in PT-type strains nos. 5598 and 5601 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain TOP10F' | Perilla frutescens var. hirtella |
recombinant His-tagged enzyme from Escherichia coli strain TOP10F' | Perilla frutescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young | Perilla frutescens var. hirtella | - |
leaf | young | Perilla frutescens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella | (-)-isopiperitenone + NADH + H+ | - |
r | |
(-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens | (-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella | (-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens 5598 | (-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella 5712 | (-)-isopiperitenone + NADH + H+ | - |
ir | |
(-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella 5712 | (-)-isopiperitenone + NADH + H+ | - |
r | |
(-)-trans-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella | (-)-isopiperitenone + NADH + H+ | - |
r | |
(-)-trans-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella 5712 | (-)-isopiperitenone + NADH + H+ | - |
r | |
additional information | ISPD1 has broader substrate specificity than ISPD2 | Perilla frutescens var. hirtella | ? | - |
? | |
additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens | ? | - |
? | |
additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens var. hirtella | ? | - |
? | |
additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens 5598 | ? | - |
? | |
additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens var. hirtella 5712 | ? | - |
? | |
additional information | ISPD1 has broader substrate specificity than ISPD2 | Perilla frutescens var. hirtella 5712 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isopiperitenol dehydrogenase 1 | - |
Perilla frutescens var. hirtella |
isopiperitenol dehydrogenase 2 | - |
Perilla frutescens |
isopiperitenol dehydrogenase 2 | - |
Perilla frutescens var. hirtella |
ISPD1 | - |
Perilla frutescens var. hirtella |
ISPD2 | - |
Perilla frutescens |
ISPD2 | - |
Perilla frutescens var. hirtella |
PK-ISPD1 | - |
Perilla frutescens var. hirtella |
PK-ISPD2 | - |
Perilla frutescens var. hirtella |
PT-ISPD2 | - |
Perilla frutescens |
PT-ISPD2 | - |
Perilla frutescens var. hirtella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Perilla frutescens var. hirtella | |
NAD+ | - |
Perilla frutescens | |
NADH | - |
Perilla frutescens var. hirtella |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthetic pathways of oil constituents (PA-type, PT-type, and PK-type) in Perilla, putative pathways, overview | Perilla frutescens var. hirtella |
metabolism | the enzyme is involved in the biosynthetic pathways of oil constituents (PA-type, PT-type, and PK-type) in Perilla, putative pathways, overview. (-)-cis-Isopiperitenol is an intermediate for the piperitenone biosynthesis of perilla | Perilla frutescens |
metabolism | the enzyme is involved in the biosynthetic pathways of oil constituents (PA-type, PT-type, and PK-type) in Perilla, putative pathways, overview. (-)-cis-Isopiperitenol is an intermediate for the piperitenone biosynthesis of perilla | Perilla frutescens var. hirtella |
physiological function | isozyme ISPD1 is less likely to be involved in oxidation of isopiperitenol | Perilla frutescens var. hirtella |
physiological function | isozyme ISPD2 is likely to be involved in oxidation of isopiperitenol | Perilla frutescens |
physiological function | isozyme ISPD2 is likely to be involved in oxidation of isopiperitenol | Perilla frutescens var. hirtella |