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Literature summary for 1.1.1.224 extracted from

  • Everard, J.D.; Cantini, C.; Grumet, R.; Plummer, J.; Loescher, W.H.
    Molecular cloning of mannose-6-phosphate reductase and its developmental expression in celery (1997), Plant Physiol., 113, 1427-1435.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.224

Cloned(Commentary)

Cloned (Comment) Organism
-
 Apium graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 NADPH enzyme purified from leaves Apium graveolens
0.0062
-
 NADPH recombinant enzyme Apium graveolens
10.1
-
 D-mannose 6-phosphate recombinant enzyme Apium graveolens
13.6
-
 D-mannose 6-phosphate enzyme purified from leaves Apium graveolens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, calculated from cDNA sequence Apium graveolens

Organism

Organism UniProt Comment Textmining
Apium graveolens
-
 celery
-
Apium graveolens
-
 cv. Giant Pascal
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme Apium graveolens

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon 21-d old, low activity Apium graveolens
-
flower low activity Apium graveolens
-
leaf
-
 Apium graveolens
-
additional information no activity in sink tissue, i.e. young root, young petiole, young leaf and mature petiole Apium graveolens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 measurement of activity during leaf development Apium graveolens
3.93
-
 purified recombinant enzyme Apium graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannose 6-phosphate + NADPH
-
 Apium graveolens D-mannitol 1-phosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, calculated from cDNA sequence Apium graveolens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Apium graveolens