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Literature summary for 1.1.1.231 extracted from

  • Korff, J.M.; Jarabak, J.
    Isolation and properties of an NADP+-dependent PGI2-specific 15-hydroxyprostaglandin dehydrogenase from rabbit kidney (1982), Methods Enzymol., 86, 152-155.
    View publication on PubMed

General Stability

General Stability Organism
freezing and thawing: repeatedly, slow loss of activity Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
NADP+
-
Oryctolagus cuniculus
0.278
-
prostaglandin I2
-
Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ Oryctolagus cuniculus
-
(5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
19.9
-
-
Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
stable for months when frozen Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+
-
Oryctolagus cuniculus (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH
-
?
(5Z,13E)-(15S)-6,9alpha-epoxy-11alpha,15-dihydroxyprosta-5,13-dienoate + NADP+ i.e. prostaglandin I2, prostacyclin Oryctolagus cuniculus (5Z,13E)-6,9alpha-epoxy-11alpha-hydroxy-15-oxoprosta-5,13-dienoate + NADPH i.e. 15-keto prostaglandin I2 ?
6-keto-prostaglandin F1alpha + NADP+ at 1.8% the reaction rate of prostaglandin I2 Oryctolagus cuniculus 6,15-diketoprostaglandin F2alpha + NADPH
-
?
additional information enzyme catalyzes reduction of 9-keto group in prostaglandin E2 at less than 1% the rate of prostaglandin I2 oxidation Oryctolagus cuniculus ?
-
?
prostaglandin B1 + NADP+ at 8% the reaction rate of prostaglandin I2 Oryctolagus cuniculus 15-ketoprostaglandin B1 + NADPH + H+
-
?
prostaglandin E2 + NADP+ at 4% the reaction rate of prostaglandin I2 Oryctolagus cuniculus 15-ketoprostaglandin E2 + NADPH + H+
-
?
prostaglandin F2alpha + NADP+ at 2.2% the reaction rate of prostaglandin I2 Oryctolagus cuniculus 15-ketoprostaglandin F2alpha + NADPH + H+
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.3
-
assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NAD+ Oryctolagus cuniculus
NADP+
-
Oryctolagus cuniculus