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Literature summary for 1.1.1.236 extracted from
- Site-directed mutagenesis of putative substrate-binding residues reveals a mechanism controlling the different stereospecificities of two tropinone reductases (1999), J. Biol. Chem., 274, 16563-16568.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Datura stramonium |
- |
Hyoscyamus niger |
| full-length sequences determined of 5 clones with high homology to TRs | Arabidopsis sp. |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Datura stramonium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00172 | - |
NADPH | mutant S148A | Datura stramonium |  |
| 0.00377 | - |
NADPH | mutant Y100H/S148A/E156V/L210I/L213F | Datura stramonium | |
| 0.00405 | - |
NADPH | mutant Y100H/S148A | Datura stramonium | |
| 0.00417 | - |
NADPH | mutant L210I/L213F | Datura stramonium | |
| 0.00817 | - |
NADPH | mutant S148A/E156V | Datura stramonium | |
| 0.0112 | - |
NADPH | mutant Y100H | Datura stramonium | |
| 0.0124 | - |
NADPH | mutantY100H/S148A/E156V | Datura stramonium | |
| 0.0187 | - |
NADPH | wild-type | Datura stramonium | |
| 0.0201 | - |
NADPH | mutant E156V | Datura stramonium | |
| 0.0255 | - |
NADPH | mutant E156V/Y100H | Datura stramonium | |
| 0.0448 | - |
tropinone | mutant L210I/L213F | Datura stramonium | |
| 0.0476 | - |
tropinone | wild-type | Datura stramonium | |
| 0.31 | - |
tropinone | mutant S148A | Datura stramonium | |
| 4.56 | - |
tropinone | mutant Y100H | Datura stramonium | |
| 6.56 | - |
tropinone | mutant S148A/E156V | Datura stramonium | |
| 19.6 | - |
tropinone | mutant Y100H/S148A | Datura stramonium | |
| 28.7 | - |
tropinone | mutant E156V | Datura stramonium | |
| 38.1 | - |
tropinone | mutant Y100H/S148A/E156V | Datura stramonium | |
| 52.5 | - |
tropinone | mutant E156V/Y100H | Datura stramonium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropinone + NADPH + H+ | Datura stramonium | - |
pseudotropine + NADP+ | - |
? | |
| tropinone + NADPH + H+ | Hyoscyamus niger | - |
pseudotropine + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis sp. | - |
- |
- |
| Datura stramonium | - |
- |
- |
| Hyoscyamus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Datura stramonium |
- |
Hyoscyamus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
? | |
| tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Datura stramonium |
| dimer | - |
Hyoscyamus niger |
| tetramer | - |
Hyoscyamus niger |
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Release 2023.1 (January 2023)
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