General Stability | Organism |
---|---|
activity rapidly lost during purification | Datura stramonium |
activity rapidly lost during purification | Atropa belladonna |
activity rapidly lost during purification | Hyoscyamus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoic acid | - |
Hyoscyamus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
NADPH | - |
Hyoscyamus niger | |
0.0076 | - |
NADH | - |
Hyoscyamus niger | |
0.017 | - |
NADPH | - |
Datura stramonium | |
0.0211 | - |
NADPH | - |
Hyoscyamus niger | |
0.023 | - |
NADPH | - |
Datura stramonium | |
0.034 | - |
tropinone | - |
Hyoscyamus niger | |
0.0351 | - |
tropinone | - |
Hyoscyamus niger | |
0.22 | - |
tropinone | - |
Datura stramonium | |
0.251 | - |
NADP+ | - |
Hyoscyamus niger | |
0.265 | - |
N-propylyl-4-piperidone | - |
Hyoscyamus niger | |
0.534 | - |
4-ethylcyclohexanone | - |
Hyoscyamus niger | |
0.77 | - |
N-Methyl-4-piperidone | - |
Hyoscyamus niger | |
0.83 | - |
tropinone | - |
Datura stramonium | |
2.03 | - |
4-methylcyclohexanone | - |
Hyoscyamus niger | |
7.58 | - |
3-methylcyclohexanone | - |
Hyoscyamus niger | |
21.6 | - |
4-Piperidone | - |
Hyoscyamus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 55000 | TRII preparation is not yet pure and shows numerous bands | Datura stramonium |
29000 | - |
3 * 29000, SDS-PAGE | Hyoscyamus niger |
29000 | - |
4 * 29000, SDS-PAGE | Hyoscyamus niger |
69000 | - |
minor peak, gel filtration | Hyoscyamus niger |
103000 | - |
major peak, gel filtration | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Datura inoxia | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Datura stramonium | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Atropa belladonna | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus muticus | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus pusillus | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus canariensis | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus bohemicus | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Physochlaina orientalis | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Physalis philadelphica | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Physalis edulis | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Calystegia sepium | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Alkekengi officinarum | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus niger | reverse reaction very inefficient, about 1.3% of the forward activity | pseudotropine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkekengi officinarum | - |
- |
- |
Atropa belladonna | - |
- |
- |
Calystegia sepium | - |
- |
- |
Datura inoxia | - |
- |
- |
Datura stramonium | - |
- |
- |
Hyoscyamus bohemicus | - |
- |
- |
Hyoscyamus canariensis | - |
- |
- |
Hyoscyamus muticus | - |
- |
- |
Hyoscyamus niger | - |
- |
- |
Hyoscyamus pusillus | - |
- |
- |
no activity in Brassica campestris | - |
- |
- |
no activity in Browallia americana | - |
- |
- |
no activity in Nicotiana tabacum | - |
tobacco | - |
Physalis edulis | - |
- |
- |
Physalis philadelphica | - |
- |
- |
Physochlaina orientalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Datura stramonium |
- |
Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Atropa belladonna | - |
leaf | - |
Atropa belladonna | - |
root | - |
Datura inoxia | - |
root | - |
Atropa belladonna | - |
root | - |
Hyoscyamus niger | - |
root | - |
Physalis philadelphica | - |
root | - |
Physalis edulis | - |
root | - |
Calystegia sepium | - |
root | - |
Alkekengi officinarum | - |
root | hairy roots | Datura stramonium | - |
stem | - |
Datura stramonium | - |
stem | - |
Atropa belladonna | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.79 | - |
- |
Datura stramonium |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 3 months | Datura stramonium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | 2-methylcyclohexanol + NADP+ | - |
? | |
3-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | 3-methylcyclohexanol + NADP+ | - |
? | |
4-ethyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-ethylcyclohexanol + NADP+ | - |
? | |
4-ethyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | 4-ethylcyclohexanol + NADP+ | - |
? | |
4-methyl-cyclohexanone + NADPH | - |
Datura stramonium | 4-methylcyclohexanol + NADP+ | - |
? | |
4-methyl-cyclohexanone + NADPH | - |
Hyoscyamus niger | 4-methylcyclohexanol + NADP+ | - |
? | |
4-piperidone + NADPH | - |
Hyoscyamus niger | piperidine-4-ol + NADP+ | - |
? | |
6-hydroxytropinone + NADPH | - |
Datura stramonium | ? + NADP+ | - |
? | |
6-hydroxytropinone + NADPH | - |
Hyoscyamus niger | ? + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Datura stramonium | N-methylpiperidin-4-ol + NADP+ | - |
? | |
N-methyl-4-piperidone + NADPH | - |
Hyoscyamus niger | N-methylpiperidin-4-ol + NADP+ | - |
? | |
N-propyl-4-piperidone + NADPH | - |
Datura stramonium | N-propylpiperidin-4-ol + NADP+ | - |
? | |
N-propyl-4-piperidone + NADPH | - |
Hyoscyamus niger | N-propylpiperidin-4-ol + NADP+ | - |
? | |
tetrahydrothiopyran-4-one + NADPH | - |
Datura stramonium | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
tetrahydrothiopyran-4-one + NADPH | - |
Hyoscyamus niger | tetrahydrothiopyran-4-ol + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Datura inoxia | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Atropa belladonna | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus muticus | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus pusillus | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus canariensis | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus bohemicus | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Physochlaina orientalis | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Physalis philadelphica | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Physalis edulis | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Calystegia sepium | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Alkekengi officinarum | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | reverse reaction very inefficient, about 1.3% of the forward activity | Hyoscyamus niger | pseudotropine + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 29000, SDS-PAGE | Hyoscyamus niger |
trimer | - |
Datura stramonium |
trimer | - |
Atropa belladonna |
trimer | 3 * 29000, SDS-PAGE | Hyoscyamus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | 6.5 | - |
Hyoscyamus niger |
5.8 | - |
forward reaction | Hyoscyamus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | reverse oxidative activities | Hyoscyamus niger |
4.5 | 8.5 | tolerant to pH changes over a wide range | Datura stramonium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.5 | more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5 | Hyoscyamus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Hyoscyamus niger | |
NADPH | activity exclusively with NADPH, not active with NADH | Datura stramonium |