Cloned (Comment) | Organism |
---|---|
gene trII, DNA and amino acid sequence determination and analysis, expression as C- or N-terminally His-tagged enzyme in Escherichia coli strain SG13009, the His-tage posotion influences the pH dependence of the enzyme, oveview | Solanum dulcamara |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the recombinant His-tagged enzyme shows an increased substrate affinity compared to the wild-type enzyme | Solanum dulcamara |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Solanum dulcamara |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Solanum dulcamara | the enzyme is involved in tropane alkaloid biosynthesis, overview | pseudotropine + NADP+ | leading to formation of calystegines | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum dulcamara | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Solanum dulcamara |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with quinuclidione and 8-thiabicyclo[3.2.1]octan-2-one | Solanum dulcamara | ? | - |
? | |
tropinone + NADPH + H+ | the enzyme is involved in tropane alkaloid biosynthesis, overview | Solanum dulcamara | pseudotropine + NADP+ | leading to formation of calystegines | ? | |
tropinone + NADPH + H+ | active site structure and catalytic triad, the recombinant His-tagged enzyme shows 4fold higher activity compared to the wild-type enzyme, overview | Solanum dulcamara | pseudotropine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Solanum dulcamara |
More | structure modelling, overview | Solanum dulcamara |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the family of short chain dehydrogenases | Solanum dulcamara |
pseudotropinone forming tropinone reductase | - |
Solanum dulcamara |
TRII | - |
Solanum dulcamara |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Solanum dulcamara |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
recombinant His-tagged enzyme | Solanum dulcamara |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant His-tagged enzyme shows a broader pH spectrum compared to the wild-type enzyme | Solanum dulcamara |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | binding site sequence, overview | Solanum dulcamara |