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Literature summary for 1.1.1.239 extracted from

  • Itagaki, E.; Iwaya, T.
    Purification and characterization of 17beta-hydroxysteroid dehydrogenase from Cylindrocarpon radicicola (1988), J. Biochem., 103, 1039-1044.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
NAD+ reduction of androstendione, pH 7.0 Ilyonectria destructans
0.0095
-
testosterone oxidation of testosterone, pH 10.0 Ilyonectria destructans
0.014
-
testosterone oxidation of testosterone, pH 7.4 Ilyonectria destructans
0.024
-
androstendione reduction of androstendione, pH 7.0 Ilyonectria destructans
0.198
-
NAD+ oxidation of testosterone, pH 10.0 Ilyonectria destructans
0.215
-
NAD+ oxidation of testosterone, pH 7.4 Ilyonectria destructans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
2 * 26000, SDS-PAGE Ilyonectria destructans
59000
-
gel filtration, native PAGE Ilyonectria destructans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
testosterone + NAD+ Ilyonectria destructans
-
androst-4-ene-3,17-dione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Ilyonectria destructans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, gelfiltration, phenyl Sepharose Ilyonectria destructans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84.6
-
-
Ilyonectria destructans

Storage Stability

Storage Stability Organism
-80°C, 30 mM Tris-HCl, pH 8.4, 1 mM EDTA, 1 mM dithiothreitol, several months, no loss of activity Ilyonectria destructans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta-hydroxytestosterone + NAD+ 76% of testosterone oxidation rate Ilyonectria destructans 11beta-hydroxyandrost-4-ene-3,17-dione + NADH
-
r
estradiol + NAD+ 46% of testosterone oxidation rate Ilyonectria destructans estrone + NADH
-
r
testosterone + NAD+
-
Ilyonectria destructans androst-4-ene-3,17-dione + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Ilyonectria destructans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
half life of 10 min at pH 10.0, no decrease in activity in the presence of 0.2 mM testosterone, 10% activity lost after 30 min at pH 7.0 Ilyonectria destructans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
oxidation of testosterone, equal velocity of oxidation and reduction at pH 6.5 Ilyonectria destructans
10
-
reduction of androstendione Ilyonectria destructans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11.6
-
Ilyonectria destructans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Ilyonectria destructans