KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
NAD+ | reduction of androstendione, pH 7.0 | Ilyonectria destructans | |
0.0095 | - |
testosterone | oxidation of testosterone, pH 10.0 | Ilyonectria destructans | |
0.014 | - |
testosterone | oxidation of testosterone, pH 7.4 | Ilyonectria destructans | |
0.024 | - |
androstendione | reduction of androstendione, pH 7.0 | Ilyonectria destructans | |
0.198 | - |
NAD+ | oxidation of testosterone, pH 10.0 | Ilyonectria destructans | |
0.215 | - |
NAD+ | oxidation of testosterone, pH 7.4 | Ilyonectria destructans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Ilyonectria destructans |
59000 | - |
gel filtration, native PAGE | Ilyonectria destructans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
testosterone + NAD+ | Ilyonectria destructans | - |
androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ilyonectria destructans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, gelfiltration, phenyl Sepharose | Ilyonectria destructans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84.6 | - |
- |
Ilyonectria destructans |
Storage Stability | Organism |
---|---|
-80°C, 30 mM Tris-HCl, pH 8.4, 1 mM EDTA, 1 mM dithiothreitol, several months, no loss of activity | Ilyonectria destructans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11beta-hydroxytestosterone + NAD+ | 76% of testosterone oxidation rate | Ilyonectria destructans | 11beta-hydroxyandrost-4-ene-3,17-dione + NADH | - |
r | |
estradiol + NAD+ | 46% of testosterone oxidation rate | Ilyonectria destructans | estrone + NADH | - |
r | |
testosterone + NAD+ | - |
Ilyonectria destructans | androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Ilyonectria destructans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
half life of 10 min at pH 10.0, no decrease in activity in the presence of 0.2 mM testosterone, 10% activity lost after 30 min at pH 7.0 | Ilyonectria destructans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
oxidation of testosterone, equal velocity of oxidation and reduction at pH 6.5 | Ilyonectria destructans |
10 | - |
reduction of androstendione | Ilyonectria destructans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11.6 | - |
Ilyonectria destructans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Ilyonectria destructans |