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Literature summary for 1.1.1.239 extracted from
- Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that may explain its over-expression in breast cancer (2010), J. Steroid Biochem. Mol. Biol., 118, 177-187.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | establishment of MCF-7 cells that stably express AKR1C3 to model its over-expression in breast cancer. AKR1C3 expression increases steroid conversion by MCF-7 cells, leading to a pro-estrogenic state. Estrone is reduced fastest by MCF-7 cells expressing AKR1C3 when compared to other substrates at 0.1M. MCF-7 cells expressing AKR1C3 cells proliferate three times faster than parental cells in response to estrone and 17-estradiol. MCF-7 cells expressing AKR1C3 cells also reduce PGD2, limiting its dehydration to form PGJ2 products | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in MCF-7 cell | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
estrone | pH 7.0, 37°C | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| MCF-7 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstenedione + NADPH + H+ | - |
Homo sapiens | testosterone + NADP+ | - |
? | |
| estrone + NADPH + H+ | - |
Homo sapiens | 17beta-estradiol + NADP+ | - |
? | |
| progesterone + NADPH + H+ | - |
Homo sapiens | 20alpha-hydroxyprogesterone + NADP+ | - |
? | |
| prostaglandin D2 + NADPH + H+ | - |
Homo sapiens | 9alpha,11beta-prostaglandin F2 + NADP+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
estrone | pH 7.0, 37°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.127 | - |
estrone | pH 7.0, 37°C | Homo sapiens | |
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