Protein Variants | Comment | Organism |
---|---|---|
additional information | Engineering the biological conversion of methanol to specialty chemicals in Escherichia coli strain BW25113 DELTAfrmA by expressing the methanol dehydrogenase from Bacillus stearothermophilus and and ribulose monophosphate (RuMP) pathway enzymes from Bacillus methanolicus. The recombinant Escherichia coli strain converts methanol into biomass components. Effective methanol assimilation by the engineered Escherichia coli strain can be enhanced in the presence of small amounts of yeast extract or tryptone. Method, overview | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Geobacillus stearothermophilus | - |
formaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | - |
Geobacillus stearothermophilus | formaldehyde + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BsMdh | - |
Geobacillus stearothermophilus |
MDH | - |
Geobacillus stearothermophilus |
NAD-dependent MDH | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermodynamics for NAD-dependent methanol oxidation are unfavorable at low temperatures but become more favorable at higher temperatures | Geobacillus stearothermophilus |
37 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus |
General Information | Comment | Organism |
---|---|---|
metabolism | assimilation of methanol into central metabolism, overview | Geobacillus stearothermophilus |