Cloned (Comment) | Organism |
---|---|
quantitative RT-PCR enzyme expression analysis, the gene expression levels of COR in tetraploids are not remarkably changed compared to diploid plants. In the roots both genes COR and CODM, downstream of thebaine in the alkaloid pathway, are downregulated in tetraploids | Papaver bracteatum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the in vitro tetraploidy induction through methyl jasmonate (MeJA) elicitation causes a significantly increased expression of norcoclaurine synthase (NCS) and salutaridinol (SAT), and a significantly decreased expression of berberine bridge enzyme (BBE) in the leaves. In the root tissues, the BBE, NCS, and SAT show an increased expression in tetraploid plants, while codeinone reductase (COR) shows a decreased expression. A similar alteration pattern is found in mixoploid plants when compared to their diploid counterparts. MeJA at concentrations of 0.1 and 0.5 mM causes a remarkable increase in the thebaine content in the roots of treated plants, where the highest thebaine content is identified in plants elicited with 0.5 mM MeJA. Elicitation treatment causes a substantial increase in the expression of NCS and SAT in the leaves, while it has no major effect on BBE, codeine 3-O-demethylase (CODM) and COR. Expression analysis in the roots shows that MeJA causes a significant increase in the expression of only BBE and NCS, while expression of other studied genes remains unchanged | Papaver bracteatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeinone + NADPH + H+ | Papaver bracteatum | - |
codeine + NADP+ | - |
r | |
morphinone + NADPH + H+ | Papaver bracteatum | - |
morphine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver bracteatum | A0A0H4BD81 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Papaver bracteatum | - |
additional information | 15 autotetraploid, 15 mixoploid, and their 30 diploid progenitors are selected after their ploidy state is reconfirmed by flow cytometry method | Papaver bracteatum | - |
root | - |
Papaver bracteatum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeinone + NADPH + H+ | - |
Papaver bracteatum | codeine + NADP+ | - |
r | |
morphinone + NADPH + H+ | - |
Papaver bracteatum | morphine + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
codeinone reductase | - |
Papaver bracteatum |
COR | - |
Papaver bracteatum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Papaver bracteatum | |
NADPH | - |
Papaver bracteatum |
Organism | Comment | Expression |
---|---|---|
Papaver bracteatum | methyl jasmonate treatment suppresses expression of codeinone reductase (COR) in roots of in tetraploid and mixoploid Papaver bracteatum plants, while the expression level of COR in leaves is unaffected by up to 0.5 mM methyl jasmonate | down |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the downstream metabolization of thebaine, benzylisoquinoline alkaloids pathway, overview. In the roots both genes COR and CODM, downstream of thebaine in the alkaloid pathway, are downregulated in tetraploids. The gene expression levels of COR in tetraploids are not remarkably changed compared to diploid plants. Via methyljasmonate treatment, gene expression of genes upstream of thebaine is upregulated while the genes downstream of thebaine are partly downregulated in tetraploids | Papaver bracteatum |