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Literature summary for extracted from

  • Fossati, E.; Narcross, L.; Ekins, A.; Falgueyret, J.P.; Martin, V.J.
    Synthesis of morphinan alkaloids in Saccharomyces cerevisiae (2015), PLoS ONE, 10, e0124459 .
    View publication on PubMedView publication on EuropePMC


Cloned (Comment) Organism
salutaridine synthase (PsSAS), salutaridine reductase (PsSAR) and salutaridinol acetyltransferase (PsSAT) are functionally coexpressed in Saccharomyces cerevisiae and optimization of the pH conditions allowed for productive spontaneous rearrangement of salutaridinol-7-O-acetate and synthesis of thebaine from (R)-reticuline. A 7-gene pathway for the production of codeine and morphine from (R)-reticuline is reconstituted. Yeast cell feeding assays using (R)-reticuline, salutaridine or codeine as substrates show that all enzymes are functionally coexpressed in yeast and that activity of salutaridine reductase and codeine-O-demethylase likely limit flux to morphine synthesis. The results of this study describe a significant advance for the synthesis of morphinans in Saccharomyces cerevisiae and pave the way for their complete synthesis in recombinant microbes Papaver somniferum


Organism UniProt Comment Textmining
Papaver somniferum Q071N0

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salutaridinol + NADP+
Papaver somniferum salutaridine + NADPH + H+


Synonyms Comment Organism
Papaver somniferum