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Literature summary for 1.1.1.248 extracted from
- Comparative transcript and alkaloid profiling in Papaver species identifies a short chain dehydrogenase/reductase involved in morphine biosynthesis (2006), Plant J., 48, 177-192.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene salR, DNA and amino acid sequence determination and analysis, expression profile, overexpression in Escherichia coli strain SG13009 as His6-tagged enzyme | Papaver somniferum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, recombinant enzyme | Papaver somniferum | |
| 0.023 | - |
DNA with apurinic site | pH 9.5, 30°C, recombinant enzyme, oxidation reaction | Papaver somniferum | |
| 0.031 | - |
salutaridine | pH 6.0, 30°C, recombinant enzyme, reduction reaction | Papaver somniferum |  |
| 0.08 | - |
NADPH | pH 6.0, 30°C, recombinant enzyme, reduction reaction | Papaver somniferum | |
| 0.198 | - |
NADP+ | pH 9.5, 30°C, recombinant enzyme, oxidation reaction | Papaver somniferum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34100 | - |
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE | Papaver somniferum |
| 40000 | - |
1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE | Papaver somniferum |
| 47100 | 50100 | recombinant enzyme, gel filtration | Papaver somniferum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Papaver somniferum | alkaloid composition of the organism | ? | - |
? | |
| additional information | Papaver somniferum salR | alkaloid composition of the organism | ? | - |
? | |
| salutaridine + NADPH + H+ | Papaver somniferum | a step in the unique biosynthesis of morphine via codeine, overview | (7S)-salutaridinol + NADP+ | - |
r | |
| salutaridine + NADPH + H+ | Papaver somniferum salR | a step in the unique biosynthesis of morphine via codeine, overview | (7S)-salutaridinol + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Papaver somniferum | Q071N0 | gene salR; salR | - |
| Papaver somniferum salR | Q071N0 | gene salR; salR | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain SG13009 | Papaver somniferum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | alkaloid composition of the organism | Papaver somniferum | ? | - |
? | |
| additional information | no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol | Papaver somniferum | ? | - |
? | |
| additional information | alkaloid composition of the organism | Papaver somniferum salR | ? | - |
? | |
| additional information | no reduction of 1,2-nordehydroreticuline, 1,2-dehydroreticulinium ion, codeinone, tropinone, or (-)-menthone, no oxidation of (R)-norreticuline, (R)-reticuline, codeine, tropine, or (+)-neomenthol | Papaver somniferum salR | ? | - |
? | |
| salutaridine + NADPH + H+ | a step in the unique biosynthesis of morphine via codeine, overview | Papaver somniferum | (7S)-salutaridinol + NADP+ | - |
r | |
| salutaridine + NADPH + H+ | NADPH-dependent reduction of the 7-keto group | Papaver somniferum | (7S)-salutaridinol + NADP+ | no formation of 7-epi-salutaridinol | r | |
| salutaridine + NADPH + H+ | a step in the unique biosynthesis of morphine via codeine, overview | Papaver somniferum salR | (7S)-salutaridinol + NADP+ | - |
r | |
| salutaridine + NADPH + H+ | NADPH-dependent reduction of the 7-keto group | Papaver somniferum salR | (7S)-salutaridinol + NADP+ | no formation of 7-epi-salutaridinol | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34100, sequence calculation, 1 * 40000, recombinant His6-tagged enzyme, SDS-PAGE | Papaver somniferum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the family of short-chain dehydrogenases/reductase | Papaver somniferum |
| SalR | - |
Papaver somniferum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | recombinant enzyme, reduction reaction | Papaver somniferum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | recombinant enzyme, 50% of maximal activity at 20°C and 40°C, reduction reaction | Papaver somniferum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | recombinant enzyme, reduction reaction | Papaver somniferum |
| 9.5 | - |
oxidation reaction, recombinant enzyme | Papaver somniferum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | 7.5 | sharp increase in activity at pH 4.7, half-maximal activity at pH 7.5, inactive at pH 9.0, recombinant enzyme, reduction reaction | Papaver somniferum |
| 9 | 10 | maximal activity at pH 9.5, sharp decline in activity at pH 10.0, oxidation reaction, recombinant enzyme | Papaver somniferum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | 5% of the activity with NADP+, oxidation reaction, recombinant enzyme | Papaver somniferum | |
| NADH | 20% of the activity with NADPH, reduction reaction, recombinant enzyme | Papaver somniferum | |
| NADP+ | preferred cofactor, oxidation reaction, recombinant enzyme | Papaver somniferum | |
| NADPH | preferred cofactor, reduction reaction, recombinant enzyme | Papaver somniferum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Papaver somniferum | amino acid sequence calculation | - |
4.7 |
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