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Literature summary for extracted from

  • Kempe, K.; Higashi, Y.; Frick, S.; Sabarna, K.; Kutchan, T.M.
    RNAi suppression of the morphine biosynthetic gene salAT and evidence of association of pathway enzymes (2009), Phytochemistry, 70, 579-589.
    View publication on PubMed


Cloned (Comment) Organism
cloned into the pMyr vector and expressed as fusion protein with a myristylation sequence, which anchors the target protein to the yeast membrane. Fusion proteins (pSOS/SalAT and pMyr/SalR) coexpressed in the Saccharomyces cerevisiae cdc25H strain. SalR amplified from the recombinant plasmid SalR/pQE-30. The 0.9-kb DNA fragment ligated into an NheI/EcoRI-digested pET28a expression vector. SalR/pET28a expression construct transformed into Escherichia coli BL21(DE3)RIL Papaver somniferum


Inhibitors Comment Organism Structure
additional information direct influence of a reduction in salAT transcript level by RNAi on the transcription of salR is not evident Papaver somniferum


Localization Comment Organism GeneOntology No. Textmining
Papaver somniferum 5829


Organism UniProt Comment Textmining
Papaver somniferum

Purification (Commentary)

Purification (Comment) Organism
His-tagged SalR purified with a cobalt affinity resin Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
Papaver somniferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salutaridine + NADPH + H+ interaction between SalR and SalAT in morphine biosynthesis Papaver somniferum (7S)-salutaridinol + NADP+


Synonyms Comment Organism
Papaver somniferum


Cofactor Comment Organism Structure
Papaver somniferum