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Literature summary for 1.1.1.248 extracted from

  • Gerardy, R.; Zenk, M.H.
    Purification and characterization of salutaridine:NADPH 7-oxidoreductase from Papaver somniferum (1993), Phytochemistry, 34, 125-132.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
salutaridinol
-
Papaver somniferum
0.023
-
salutaridine
-
Papaver somniferum
0.055
-
NADP+
-
Papaver somniferum
0.125
-
NADPH
-
Papaver somniferum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Papaver somniferum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
gel filtration Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
salutaridine + NADPH + H+ Papaver somniferum formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids salutaridinol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
no activity in Papaver alpinum
-
-
-
no activity in Papaver atlanticum
-
-
-
no activity in Papaver dubium
-
-
-
no activity in Papaver feddei
-
-
-
no activity in Papaver lateritium
-
-
-
no activity in Papaver oreophilum
-
-
-
no activity in Papaver orientale
-
-
-
no activity in Papaver persicum
-
-
-
no activity in Papaver pilosum
-
-
-
no activity in Papaver rhoeas
-
-
-
no activity in Papaver rupifragum
-
-
-
no activity in Papaver strigosum
-
-
-
no activity in Papaver tauricula
-
-
-
no activity in Papaver triniifolium
-
-
-
Papaver bracteatum
-
-
-
Papaver somniferum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Papaver somniferum
-
seedling
-
Papaver somniferum
-
additional information no activity in leaves Papaver somniferum
-
cell suspension culture
-
Papaver somniferum
-
shoot
-
Papaver somniferum
-
plant capsule
-
Papaver somniferum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Papaver somniferum

Storage Stability

Storage Stability Organism
4°C, pH 7.0-9.0, 0.6 M KCl, half-life: 30 days Papaver somniferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salutaridine + NADPH + H+ r Papaver somniferum (7S)-salutaridinol + NADP+
-
?
salutaridine + NADPH + H+ formation of salutaridinol, a precursor for the synthesis of morphine and related opium alkaloids Papaver somniferum salutaridinol + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 52000, SDS-PAGE Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35 both directions Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5 formation of (7S)-salutaridinol Papaver somniferum
9 9.5 formation of salutaridine Papaver somniferum

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 50% of maximal activity at pH 5.8 and at pH 7.8, formationof salutaridine Papaver somniferum
5.8 7.8 50% of maximal activity at pH 5.8 and at pH 7.8, formation of (7S)-salutaridinol Papaver somniferum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 4°C, half-life: 30 days Papaver somniferum