Cloned (Comment) | Organism |
---|---|
gene aroE, overexpression in the aroE auxotrophic mutant strain AB2834 | Escherichia coli |
overexpression in Escherichia coli K12 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
purified recombinant enzyme, 20 mg/ml in 10 mM Tris-HCl, pH 7.6, 0.4 mM DTT, and 2.5 mM NADPH, 20°C, sitting-drop vapour-diffusion method, reservoir solution contains 1.65 M ammonium sulfate, 100 mM cacodylate buffer, pH 5.8, 1.5 ml of protein solution is mixed with an equal volume of reservoir solution, 10-12 days, cryoprotection with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29400 | - |
mass spectometry and calculation from DNA sequence | Escherichia coli |
29410 | - |
mass spectrometry | Escherichia coli |
29414 | - |
1 * 29414, mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | Escherichia coli | - |
3-dehydroshikimate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
recombinant enzyme from aroE auxotrophic mutant strain AB2834 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl, pH 7.5, 0.4 mM DTT, 50% glycerol, long-term | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
shikimate + NADP+ | - |
Escherichia coli | 3-dehydroshikimate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29400, mass spectrometry and gel filtration | Escherichia coli |
monomer | 1 * 29414, mass spectrometry | Escherichia coli |
More | structure analysis, dynamic light scattering measurements | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |