BRENDA - Enzyme Database
show all sequences of 1.1.1.264

Crystallization and properties of 5-keto-D-gluconate reductase from Gluconobacter suboxydans

Adachi, O.; Shinagawa, E.; Matsushita, K.; Ameyama, M.; Agric. Biol. Chem. 43, 75-83 (1979)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
55% ammonium sulfate precipitation
Gluconobacter oxydans
General Stability
General Stability
Organism
highly unstable, 75% loss of activity within a few days, stabilizing agents during purification: D-gluconate, 5-keto-D-gluconate
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Gluconobacter oxydans
Cs2+
-
Gluconobacter oxydans
Cu2+
-
Gluconobacter oxydans
Hg2+
-
Gluconobacter oxydans
Ni2+
-
Gluconobacter oxydans
p-chloromercuribenzoate
-
Gluconobacter oxydans
Sn2+
-
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
NADPH
-
Gluconobacter oxydans
0.02
-
NADP+
-
Gluconobacter oxydans
0.9
-
5-Keto-D-gluconate
-
Gluconobacter oxydans
20
-
D-gluconate
-
Gluconobacter oxydans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
slight activation at 1 mM
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
4 * 25000, SDS-PAGE
Gluconobacter oxydans
110000
-
gel filtration
Gluconobacter oxydans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-idonate + NADP+
Gluconobacter oxydans
-
5-dehydro-D-gluconate + NADPH
-
Gluconobacter oxydans
r
Organism
Organism
UniProt
Commentary
Textmining
Gluconobacter oxydans
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity, crystalline, chromatography techniques
Gluconobacter oxydans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Gluconobacter oxydans
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
69.7
-
-
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-keto-D-fructose + NADP+
low activity
285823
Gluconobacter oxydans
? + NADPH
-
285823
Gluconobacter oxydans
?
D-gluconate + NADP+
-
285823
Gluconobacter oxydans
? + NADPH
-
285823
Gluconobacter oxydans
r
L-idonate + NADP+
-
285823
Gluconobacter oxydans
5-dehydro-D-gluconate + NADPH
-
285823
Gluconobacter oxydans
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 25000, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
5-keto-D-gluconate reduction
Gluconobacter oxydans
10
-
D-gluconate oxidation
Gluconobacter oxydans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
6
80% of maximal activity within
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Gluconobacter oxydans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
55% ammonium sulfate precipitation
Gluconobacter oxydans
General Stability (protein specific)
General Stability
Organism
highly unstable, 75% loss of activity within a few days, stabilizing agents during purification: D-gluconate, 5-keto-D-gluconate
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
-
Gluconobacter oxydans
Cs2+
-
Gluconobacter oxydans
Cu2+
-
Gluconobacter oxydans
Hg2+
-
Gluconobacter oxydans
Ni2+
-
Gluconobacter oxydans
p-chloromercuribenzoate
-
Gluconobacter oxydans
Sn2+
-
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
NADPH
-
Gluconobacter oxydans
0.02
-
NADP+
-
Gluconobacter oxydans
0.9
-
5-Keto-D-gluconate
-
Gluconobacter oxydans
20
-
D-gluconate
-
Gluconobacter oxydans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
slight activation at 1 mM
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
4 * 25000, SDS-PAGE
Gluconobacter oxydans
110000
-
gel filtration
Gluconobacter oxydans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-idonate + NADP+
Gluconobacter oxydans
-
5-dehydro-D-gluconate + NADPH
-
Gluconobacter oxydans
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, crystalline, chromatography techniques
Gluconobacter oxydans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Gluconobacter oxydans
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
69.7
-
-
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-keto-D-fructose + NADP+
low activity
285823
Gluconobacter oxydans
? + NADPH
-
285823
Gluconobacter oxydans
?
D-gluconate + NADP+
-
285823
Gluconobacter oxydans
? + NADPH
-
285823
Gluconobacter oxydans
r
L-idonate + NADP+
-
285823
Gluconobacter oxydans
5-dehydro-D-gluconate + NADPH
-
285823
Gluconobacter oxydans
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 25000, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
-
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
5-keto-D-gluconate reduction
Gluconobacter oxydans
10
-
D-gluconate oxidation
Gluconobacter oxydans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
6
80% of maximal activity within
Gluconobacter oxydans
Other publictions for EC 1.1.1.264
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670701
DeBolt
L-tartaric acid synthesis from ...
Ampelopsis glandulosa var. brevipedunculata, no activity in Ampelopsis aconitifolia, Vitis californica, Vitis vinifera
Proc. Natl. Acad. Sci. USA
103
5608-5613
2006
-
-
3
-
-
-
-
2
-
-
-
3
-
4
-
-
3
-
-
6
1
-
6
-
6
3
-
-
-
6
-
-
6
-
-
-
-
-
3
6
-
-
-
-
-
-
2
-
-
-
3
-
-
-
3
-
6
1
-
6
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
246372
Bausch
Sequence analysis of the GntII ...
Escherichia coli
J. Bacteriol.
180
3704-3710
1998
-
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285823
Adachi
-
Crystallization and properties ...
Gluconobacter oxydans
Agric. Biol. Chem.
43
75-83
1979
-
-
-
1
-
1
7
4
-
1
2
1
-
1
-
-
1
-
-
1
1
-
3
1
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
1
1
-
1
-
7
-
4
-
1
2
1
-
-
-
1
-
1
1
-
3
1
1
-
-
-
2
-
1
-
-
-
-
-
-
-
285822
Chiyonobu
-
Distribution of 2-ketogluconat ...
Acetobacter aceti, Frateuria aurantia, Gluconacetobacter liquefaciens, Gluconobacter albidus, Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter oxydans, Gluconobacter roseus, Gluconobacter sphaericus
Agric. Biol. Chem.
39
2425-2427
1975
-
-
-
-
-
-
-
-
-
-
-
9
-
9
-
-
-
-
-
9
-
-
9
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
9
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285824
Takagi
-
A new enzyme, 5-ketoglucono-id ...
Fusarium sp.
Agric. Biol. Chem.
26
719-720
1962
-
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-