BRENDA - Enzyme Database
show all sequences of 1.1.1.264

L-tartaric acid synthesis from vitamin C in higher plants

DeBolt, S.; Cook, D.R.; Ford, C.M.; Proc. Natl. Acad. Sci. USA 103, 5608-5613 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Ampelopsis glandulosa var. brevipedunculata
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Vitis californica
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Vitis vinifera
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
L-Idonate
pH 5.4, 30°C, recombinant enzyme
Vitis vinifera
12.5
-
5-Dehydro-D-gluconate
pH 8.5, 30°C, recombinant enzyme
Vitis vinifera
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-idonate + NADP+
Vitis vinifera
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
5-dehydro-D-gluconate + NADPH
-
-
r
L-idonate + NADP+
Vitis californica
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
5-dehydro-D-gluconate + NADPH
-
-
r
L-idonate + NADP+
Ampelopsis glandulosa var. brevipedunculata
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
5-dehydro-D-gluconate + NADPH
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Ampelopsis glandulosa var. brevipedunculata
-
-
-
no activity in Ampelopsis aconitifolia
-
-
-
Vitis californica
-
-
-
Vitis vinifera
Q1PSI9
cv. Cabernet Sauvignon
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)
Ampelopsis glandulosa var. brevipedunculata
recombinant enzyme from Escherichia coli strain BL21(DE3)
Vitis californica
recombinant enzyme from Escherichia coli strain BL21(DE3)
Vitis vinifera
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Ampelopsis glandulosa var. brevipedunculata
-
fruit
-
Vitis californica
-
fruit
-
Vitis vinifera
-
leaf
young
Ampelopsis glandulosa var. brevipedunculata
-
leaf
young
Vitis californica
-
leaf
young
Vitis vinifera
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
in vivo activity, feeding experiments, overview
Vitis vinifera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-idonate + NADP+
-
670701
Vitis vinifera
5-dehydro-D-gluconate + NADPH
i.e. 5-keto-D-gluconic acid
-
-
r
L-idonate + NADP+
-
670701
Vitis californica
5-dehydro-D-gluconate + NADPH
i.e. 5-keto-D-gluconic acid
-
-
r
L-idonate + NADP+
-
670701
Ampelopsis glandulosa var. brevipedunculata
5-dehydro-D-gluconate + NADPH
i.e. 5-keto-D-gluconic acid
-
-
r
L-idonate + NADP+
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
670701
Vitis vinifera
5-dehydro-D-gluconate + NADPH
-
-
-
r
L-idonate + NADP+
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
670701
Vitis californica
5-dehydro-D-gluconate + NADPH
-
-
-
r
L-idonate + NADP+
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
670701
Ampelopsis glandulosa var. brevipedunculata
5-dehydro-D-gluconate + NADPH
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
L-IdnDH
-
Ampelopsis glandulosa var. brevipedunculata
L-IdnDH
-
Vitis californica
L-IdnDH
-
Vitis vinifera
L-idonate dehydrogenase
-
Ampelopsis glandulosa var. brevipedunculata
L-idonate dehydrogenase
-
Vitis californica
L-idonate dehydrogenase
-
Vitis vinifera
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Ampelopsis glandulosa var. brevipedunculata
30
-
assay at
Vitis californica
30
-
assay at
Vitis vinifera
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
forward, oxidation reaction
Ampelopsis glandulosa var. brevipedunculata
5.4
-
forward, oxidation reaction
Vitis californica
5.4
-
forward, oxidation reaction
Vitis vinifera
8.5
-
reverse, reduction reaction
Ampelopsis glandulosa var. brevipedunculata
8.5
-
reverse, reduction reaction
Vitis californica
8.5
-
reverse, reduction reaction
Vitis vinifera
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ampelopsis glandulosa var. brevipedunculata
NADP+
-
Vitis californica
NADP+
-
Vitis vinifera
NADPH
-
Ampelopsis glandulosa var. brevipedunculata
NADPH
-
Vitis californica
NADPH
-
Vitis vinifera
Cloned(Commentary) (protein specific)
Commentary
Organism
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Ampelopsis glandulosa var. brevipedunculata
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Vitis californica
gene 1029130, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
Vitis vinifera
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ampelopsis glandulosa var. brevipedunculata
NADP+
-
Vitis californica
NADP+
-
Vitis vinifera
NADPH
-
Ampelopsis glandulosa var. brevipedunculata
NADPH
-
Vitis californica
NADPH
-
Vitis vinifera
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.2
-
L-Idonate
pH 5.4, 30°C, recombinant enzyme
Vitis vinifera
12.5
-
5-Dehydro-D-gluconate
pH 8.5, 30°C, recombinant enzyme
Vitis vinifera
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-idonate + NADP+
Vitis vinifera
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
5-dehydro-D-gluconate + NADPH
-
-
r
L-idonate + NADP+
Vitis californica
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
5-dehydro-D-gluconate + NADPH
-
-
r
L-idonate + NADP+
Ampelopsis glandulosa var. brevipedunculata
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
5-dehydro-D-gluconate + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)
Ampelopsis glandulosa var. brevipedunculata
recombinant enzyme from Escherichia coli strain BL21(DE3)
Vitis californica
recombinant enzyme from Escherichia coli strain BL21(DE3)
Vitis vinifera
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Ampelopsis glandulosa var. brevipedunculata
-
fruit
-
Vitis californica
-
fruit
-
Vitis vinifera
-
leaf
young
Ampelopsis glandulosa var. brevipedunculata
-
leaf
young
Vitis californica
-
leaf
young
Vitis vinifera
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
in vivo activity, feeding experiments, overview
Vitis vinifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-idonate + NADP+
-
670701
Vitis vinifera
5-dehydro-D-gluconate + NADPH
i.e. 5-keto-D-gluconic acid
-
-
r
L-idonate + NADP+
-
670701
Vitis californica
5-dehydro-D-gluconate + NADPH
i.e. 5-keto-D-gluconic acid
-
-
r
L-idonate + NADP+
-
670701
Ampelopsis glandulosa var. brevipedunculata
5-dehydro-D-gluconate + NADPH
i.e. 5-keto-D-gluconic acid
-
-
r
L-idonate + NADP+
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
670701
Vitis vinifera
5-dehydro-D-gluconate + NADPH
-
-
-
r
L-idonate + NADP+
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
670701
Vitis californica
5-dehydro-D-gluconate + NADPH
-
-
-
r
L-idonate + NADP+
the enzyme is involved in the biosynthesis of L-tartaric acid from ascorbic acid, pathway overview
670701
Ampelopsis glandulosa var. brevipedunculata
5-dehydro-D-gluconate + NADPH
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Ampelopsis glandulosa var. brevipedunculata
30
-
assay at
Vitis californica
30
-
assay at
Vitis vinifera
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
forward, oxidation reaction
Ampelopsis glandulosa var. brevipedunculata
5.4
-
forward, oxidation reaction
Vitis californica
5.4
-
forward, oxidation reaction
Vitis vinifera
8.5
-
reverse, reduction reaction
Ampelopsis glandulosa var. brevipedunculata
8.5
-
reverse, reduction reaction
Vitis californica
8.5
-
reverse, reduction reaction
Vitis vinifera
Other publictions for EC 1.1.1.264
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
670701
DeBolt
L-tartaric acid synthesis from ...
Ampelopsis glandulosa var. brevipedunculata, no activity in Ampelopsis aconitifolia, Vitis californica, Vitis vinifera
Proc. Natl. Acad. Sci. USA
103
5608-5613
2006
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3
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2
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3
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4
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3
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6
1
-
6
-
6
3
-
-
-
6
-
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6
-
-
-
-
-
3
6
-
-
-
-
-
-
2
-
-
-
3
-
-
-
3
-
6
1
-
6
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
246372
Bausch
Sequence analysis of the GntII ...
Escherichia coli
J. Bacteriol.
180
3704-3710
1998
-
-
1
-
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285823
Adachi
-
Crystallization and properties ...
Gluconobacter oxydans
Agric. Biol. Chem.
43
75-83
1979
-
-
-
1
-
1
7
4
-
1
2
1
-
1
-
-
1
-
-
1
1
-
3
1
-
1
-
-
-
2
-
1
1
-
-
-
-
-
-
1
1
-
1
-
7
-
4
-
1
2
1
-
-
-
1
-
1
1
-
3
1
1
-
-
-
2
-
1
-
-
-
-
-
-
-
285822
Chiyonobu
-
Distribution of 2-ketogluconat ...
Acetobacter aceti, Frateuria aurantia, Gluconacetobacter liquefaciens, Gluconobacter albidus, Gluconobacter cerinus, Gluconobacter gluconicus, Gluconobacter oxydans, Gluconobacter roseus, Gluconobacter sphaericus
Agric. Biol. Chem.
39
2425-2427
1975
-
-
-
-
-
-
-
-
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-
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9
-
9
-
-
-
-
-
9
-
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9
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
9
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285824
Takagi
-
A new enzyme, 5-ketoglucono-id ...
Fusarium sp.
Agric. Biol. Chem.
26
719-720
1962
-
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-