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Literature summary for 1.1.1.265 extracted from

  • Van Iersel, M.F.; Eppink, M.H.; van Berkel, W.J.; Rombouts, F.M.; Abee, T.
    Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae (1997), Appl. Environ. Microbiol., 63, 4079-4082.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
KCl 5-6fold increasing activity for NADPH-dependent activity at 0.2 M Saccharomyces cerevisiae
NaCl 5-6fold increasing activity for NADPH-dependent activity at 0.2 M Saccharomyces cerevisiae

Application

Application Comment Organism
nutrition essential in removal of the worthy off-flavours in beer during fermentation Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
2-methylbutanal presence of NADPH, Ki: 2.74 mM, presence of NADH, Ki: 4.61 mM Saccharomyces cerevisiae
3-Methylbutanal presence of NADPH, Ki: 7.14 mM, presence of NADH, Ki: 253 mM Saccharomyces cerevisiae
Butanal presence of NADPH, Ki: 16.6 mM Saccharomyces cerevisiae
Heptanal presence of NADPH, Ki: 0.88 mM, presence of NADH, Ki: 1.17 mM Saccharomyces cerevisiae
hexanal presence of NADPH, Ki: 0.79 mM, presence of NADH, Ki: 2.8 mM Saccharomyces cerevisiae
KCl 5-6fold decreasing activity at 0.2 M for NADH-dependent activity Saccharomyces cerevisiae
NaCl 5-6fold decreasing activity at 0.2 M for NADH-dependent activity Saccharomyces cerevisiae
NADP+ complete inhibition at a 6.7fold excess of NADP+ in a reduction assay with 0.15 mM NADH Saccharomyces cerevisiae
pentanal presence of NADPH, Ki: 3.9 mM, presence of NADH, Ki: 13.9 mM Saccharomyces cerevisiae
propanal presence of NADPH, Ki: 46.6 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADPH
-
Saccharomyces cerevisiae
0.16
-
pentanal presence of NADPH Saccharomyces cerevisiae
0.18
-
hexanal presence of NADPH Saccharomyces cerevisiae
0.21
-
3-Methylbutanal presence of NADPH Saccharomyces cerevisiae
0.27
-
Heptanal presence of NADPH Saccharomyces cerevisiae
0.83
-
hexanal presence of NADH Saccharomyces cerevisiae
1.22
-
Hexanol presence of NADP+ Saccharomyces cerevisiae
1.69
-
trans-2-hexenol presence of NADP+ Saccharomyces cerevisiae
1.7
-
NADH
-
Saccharomyces cerevisiae
1.85
-
2-methylbutanal presence of NADPH Saccharomyces cerevisiae
1.89
-
3-Methylbutanal presence of NADH Saccharomyces cerevisiae
2.76
-
Butanal presence of NADPH Saccharomyces cerevisiae
3.01
-
pentanal presence of NADH Saccharomyces cerevisiae
4.25
-
Heptanal presence of NADH Saccharomyces cerevisiae
17.7
-
2-methylbutanal presence of NADH Saccharomyces cerevisiae
23.1
-
Butanal presence of NADH Saccharomyces cerevisiae
27
-
propanal presence of NADH Saccharomyces cerevisiae
38.9
-
propanal presence of NADPH Saccharomyces cerevisiae
158
-
acetaldehyde presence of NADPH Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-methylbutanal + NAD(P)H Saccharomyces cerevisiae
-
3-methylbutanol + NAD(P)+
-
r
acetaldehyde + NADPH + H+ Saccharomyces cerevisiae
-
ethanol + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
brewer's yeast
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
61.6
-
in the presence of NADPH Saccharomyces cerevisiae
93.9
-
in the presence of NADH Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-pentanedione + NAD(P)H very low activity Saccharomyces cerevisiae ? + NAD(P)+
-
?
2-methylbutanal + NAD(P)H
-
Saccharomyces cerevisiae 2-methylbutanol + NAD(P)+
-
r
3-methylbutanal + NAD(P)H
-
Saccharomyces cerevisiae 3-methylbutanol + NAD(P)+
-
r
3-methylbutanal + NADH + H+
-
Saccharomyces cerevisiae 3-methylbutanol + NAD+
-
r
acetaldehyde + NADPH + H+
-
Saccharomyces cerevisiae ethanol + NADP+
-
r
benzaldehyde + NAD(P)H 20-50% of the activity with 3-methylbutanal for aromatic aldehydes Saccharomyces cerevisiae benzyl alcohol + NAD(P)+
-
?
butanal + NAD(P)H + H+
-
Saccharomyces cerevisiae butanol + NAD(P)+
-
r
diacetyl + NAD(P)H very low activity Saccharomyces cerevisiae ? + NAD(P)+
-
?
furaldehyde + NAD(P)H 20-50% of the activity with 3-methylbutanal for aromatic aldehydes Saccharomyces cerevisiae furfuryl alcohol + NAD(P)+
-
?
heptanal + NADPH preference for long and branched-chain substrates with up to seven carbon atoms Saccharomyces cerevisiae heptanol + NADP+
-
r
hexanal + NADPH + H+
-
Saccharomyces cerevisiae hexanol + NADP+
-
r
p-anisaldehyde + NAD(P)H 20-50% of the activity with 3-methylbutanal for aromatic aldehydes Saccharomyces cerevisiae p-anisalcohol + NAD(P)+
-
?
pentanal + NADPH
-
Saccharomyces cerevisiae pentanol + NADP+
-
r
propanal + NAD(P)H + H+
-
Saccharomyces cerevisiae propanol + NAD(P)+
-
r
trans-2-hexenol + NADP+
-
Saccharomyces cerevisiae trans-2-hexenal + NADPH
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
bcADH
-
Saccharomyces cerevisiae
branched-chain alcohol dehydrogenase
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.55
-
Hexanol in the presence of NADP+ Saccharomyces cerevisiae
5.6
-
acetaldehyde in the presence of NADPH Saccharomyces cerevisiae
14
-
trans-2-hexenol in the presence of NADP+ Saccharomyces cerevisiae
47.3
-
Butanal presence of NADH Saccharomyces cerevisiae
50.1
-
hexanal presence of NADH Saccharomyces cerevisiae
55
-
propanal presence of NADPH Saccharomyces cerevisiae
56.6
-
pentanal presence of NADPH Saccharomyces cerevisiae
57
-
Butanal presence of NADPH Saccharomyces cerevisiae
71.1
-
hexanal presence of NADPH Saccharomyces cerevisiae
72.9
-
Heptanal presence of NADPH Saccharomyces cerevisiae
74.9
-
3-Methylbutanal presence of NADPH Saccharomyces cerevisiae
81.6
-
pentanal presence of NADH Saccharomyces cerevisiae
84
-
propanal presence of NADH Saccharomyces cerevisiae
91.8
-
3-Methylbutanal presence of NADH Saccharomyces cerevisiae
92.3
-
2-methylbutanal presence of NADH Saccharomyces cerevisiae
101
-
Heptanal presence of NADH Saccharomyces cerevisiae
113
-
2-methylbutanal presence of NADPH Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 NADH-dependent activity, highest activity at low ionic strength Saccharomyces cerevisiae
8.5
-
NADPH-dependent activity, highest activity at high ionic strength Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH
-
Saccharomyces cerevisiae
NADPH preferred cofactor Saccharomyces cerevisiae