Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
in complex with NADPH and Mg2+, and in complex with fosmidomycin, NADPH and Mg2+, both to 2.0 A resolution | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosmidomycin | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
NADPH | pH 7.5, 50°C | Thermotoga maritima | |
0.0105 | - |
NADPH | pH 7.5, 85°C | Thermotoga maritima | |
0.04 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 50°C | Thermotoga maritima | |
0.11 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 85°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ is prefered over Mn2+ | Thermotoga maritima | |
Mn2+ | Mg2+ is prefered over Mn2+ | Thermotoga maritima | |
additional information | divalent cation is required | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WZZ1 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
presence of Mn2+, 50°C, pH 7.5 | Thermotoga maritima |
5 | - |
presence of Mg2+, 50°C, pH 7.5 | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Thermotoga maritima | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
optimum in presence of Mn2+ | Thermotoga maritima |
85 | - |
or above, optimum in presence of Mg2+ | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
almost complete loss of activity below | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
NADPH | pH 7.5, 50°C | Thermotoga maritima | |
5.5 | - |
NADPH | pH 7.5, 85°C | Thermotoga maritima |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
93 | - |
pH 7.5, 50°C | Thermotoga maritima | fosmidomycin | |
107 | - |
pH 7.5, 85°C | Thermotoga maritima | fosmidomycin |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 50°C | Thermotoga maritima | |
50 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.5, 85°C | Thermotoga maritima |