Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.267 extracted from

  • Takenoya, M.; Ohtaki, A.; Noguchi, K.; Endo, K.; Sasaki, Y.; Ohsawa, K.; Yajima, S.; Yohda, M.
    Crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase from the hyperthermophile Thermotoga maritima for insights into the coordination of conformational changes and an inhibitor binding (2010), J. Struct. Biol., 170, 532-539.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NADPH and Mg2+, and in complex with fosmidomycin, NADPH and Mg2+, both to 2.0 A resolution Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
fosmidomycin
-
Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
NADPH pH 7.5, 50°C Thermotoga maritima
0.0105
-
NADPH pH 7.5, 85°C Thermotoga maritima
0.04
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 50°C Thermotoga maritima
0.11
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 85°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ is prefered over Mn2+ Thermotoga maritima
Mn2+ Mg2+ is prefered over Mn2+ Thermotoga maritima
additional information divalent cation is required Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WZZ1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
presence of Mn2+, 50°C, pH 7.5 Thermotoga maritima
5
-
presence of Mg2+, 50°C, pH 7.5 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Thermotoga maritima 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
optimum in presence of Mn2+ Thermotoga maritima
85
-
or above, optimum in presence of Mg2+ Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
almost complete loss of activity below Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
NADPH pH 7.5, 50°C Thermotoga maritima
5.5
-
NADPH pH 7.5, 85°C Thermotoga maritima

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
93
-
pH 7.5, 50°C Thermotoga maritima fosmidomycin
107
-
pH 7.5, 85°C Thermotoga maritima fosmidomycin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.5
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 50°C Thermotoga maritima
50
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 85°C Thermotoga maritima