Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
W203A | complete loss of activity | Mycobacterium tuberculosis |
W203F | turnover is largely limited by product release for the wild-type enzyme, chemistry is significantly more rate-limiting for mutants W203F and W203Y. Mutant is more sensitive to fosmidomycin than wild-type. Mutation strongly tips the entropy-enthalpy balance of total binding energy | Mycobacterium tuberculosis |
W203G | complete loss of activity | Mycobacterium tuberculosis |
W203Y | turnover is largely limited by product release for the wild-type enzyme, chemistry is significantly more rate-limiting for mutants W203F and W203Y. Mutant is more sensitive to fosmidomycin than wild-type. Mutation strongly tips the entropy-enthalpy balance of total binding energy | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosmidomycin | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.137 | - |
1-deoxy-D-xylulose 5-phosphate | wild-type, pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.39 | - |
1-deoxy-D-xylulose 5-phosphate | mutant W203Y, pH 7.5, 25°C | Mycobacterium tuberculosis | |
1.015 | - |
1-deoxy-D-xylulose 5-phosphate | mutant W203F, pH 7.5, 25°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNS1 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNS1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.59 | - |
1-deoxy-D-xylulose 5-phosphate | mutant W203F, pH 7.5, 25°C | Mycobacterium tuberculosis | |
3.54 | - |
1-deoxy-D-xylulose 5-phosphate | mutant W203Y, pH 7.5, 25°C | Mycobacterium tuberculosis | |
4.2 | - |
1-deoxy-D-xylulose 5-phosphate | wild-type, pH 7.5, 25°C | Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
fosmidomycin | mutant W203Y, pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.00006 | - |
fosmidomycin | mutant W203F, pH 7.5, 25°C | Mycobacterium tuberculosis | |
0.000093 | - |
fosmidomycin | wild-type, pH 7.5, 25°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.46 | - |
1-deoxy-D-xylulose 5-phosphate | mutant W203F, pH 7.5, 25°C | Mycobacterium tuberculosis | |
9.1 | - |
1-deoxy-D-xylulose 5-phosphate | mutant W203Y, pH 7.5, 25°C | Mycobacterium tuberculosis | |
31 | - |
1-deoxy-D-xylulose 5-phosphate | wild-type, pH 7.5, 25°C | Mycobacterium tuberculosis |