Cloned (Comment) | Organism |
---|---|
gene DXR, recombinant expression of YFP-tagged enzyme DXR in Catharanthus roseus cells | Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | diffuse in stroma and stromules of plastids, immunogold labeling of DXR in the stroma of plastids from Catharanthus roseus leaf cells | Catharanthus roseus | 9507 | - |
additional information | enzyme 1-deoxy-D-xylulose 5-phosphate reductoisomerase exhibits an artifactual aggregated pattern of localization due to high protein accumulation. It is rather diffuse in stroma and stromules of plastids, subcellular localization study, MEP pathway gene RNA seq coexpression pattern, overview | Catharanthus roseus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Catharanthus roseus | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CR6 cell | - |
Catharanthus roseus | - |
leaf | all MEP pathway genes are coordinately and mainly expressed in internal phloem-associated parenchyma of young leaves | Catharanthus roseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Catharanthus roseus | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-deoxy-D-xylulose 5-phosphate reductoisomerase | - |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Catharanthus roseus | |
NADPH | - |
Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway in leaves. The MEP pathway initiates the biosynthesis of highly valuable monoterpene indole alkaloids (MIAs). The MIA biosynthetic pathway shows in leaves a complex compartmentation occuring at both the cellular and subcellular levels, notably for some gene products of the MEP pathway. All MEP pathway genes are coordinately and mainly expressed in internal phloem-associated parenchyma of young leaves, reinforcing the role of this tissue in MIA biosynthesis. A potential role of stromules in enhancing MIA precursor exchange with other cell compartments to favor metabolic fluxes towards the MIA biosynthesis. The MEP pathway produces both isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) through seven enzymatic reactions initiated by the synthesis of 1-deoxy-D-xylulose 5-phosphate (DXP) from pyruvate and glyceraldehyde 3-phosphate. This reaction is catalyzed by DXP synthase (DXS), encoded by a small gene family in higher plants. The DXS isogenes have been clustered into two related gene groups: Clade I-DXS including housekeeping genes and Clade II-DXS including genes associated with plant defense and secondary metabolism. DXP is then sequentially converted into MEP by DXP reductoisomerase (DXR) and into 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (CDP-ME) following the addition of cytidine triphosphate by CDP-ME synthase (CMS), pathway overview. MEP pathway enzymes are targeted to plastids | Catharanthus roseus |