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Literature summary for 1.1.1.27 extracted from

  • Shoemark, D.K.; Cliff, M.J.; Sessions, R.B.; Clarke, A.R.
    Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum (2007), FEBS J., 274, 2738-2748.
    View publication on PubMed
Search for more literature for 1.1.1.27

Application

Application Comment Organism
drug development parasite LDH is a target for antimalarial compounds owing to structural and functional differences from the human isozymes Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
L-lactate product inhibition Plasmodium falciparum
additional information inhibition mechanism, the plasmodial enzyme possesses a five-residue insertion in the substrate-specificity loop and exhibits less marked substrate inhibition than its mammalian counterparts, overview Plasmodium falciparum
NAD+ product inhibition Plasmodium falciparum
pyruvate the enzyme shows substrate inhibition, inhibition mechanism, overview Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state and transient kinetics, rapid kinetics of the multiple-turnover reaction, overview Plasmodium falciparum
0.011
-
 NADH pH 7.5 Plasmodium falciparum
0.047
-
 L-lactate pH 7.5 Plasmodium falciparum
0.055
-
 pyruvate pH 7.5 Plasmodium falciparum
0.116
-
 2-oxovalerate pH 7.5 Plasmodium falciparum
0.18
-
 NAD+ pH 7.5 Plasmodium falciparum
0.6
-
 2-oxobutyrate pH 7.5 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate + NADH + H+ Plasmodium falciparum
-
 (S)-lactate + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH
-
 Plasmodium falciparum 2-hydroxybutyrate + NAD+
-
 r
2-oxovalerate + NADH
-
 Plasmodium falciparum 2-hydroxyvalerate + NAD+
-
 r
pyruvate + NADH + H+
-
 Plasmodium falciparum (S)-lactate + NAD+
-
 r

Synonyms

Synonyms Comment Organism
lactate dehydrogenase
-
 Plasmodium falciparum
LDH
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.64
-
 2-oxovalerate pH 7.5 Plasmodium falciparum
40
-
 NAD+ pH 7.5 Plasmodium falciparum
40
-
 L-lactate pH 7.5 Plasmodium falciparum
80
-
 2-oxobutyrate pH 7.5 Plasmodium falciparum
96
-
 NADH pH 7.5 Plasmodium falciparum
96
-
 pyruvate pH 7.5 Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Plasmodium falciparum
NADH
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information substrate and product inhibition kinetics Plasmodium falciparum
0.14
-
 pyruvate pH 7.5 Plasmodium falciparum