Literature summary for 1.1.1.27 extracted from

Hiraka, K.; Tsugawa, W.; Asano, R.; Yokus, M.A.; Ikebukuro, K.; Daniele, M.A.; Sode, K.
Rational design of direct electron transfer type L-lactate dehydrogenase for the development of multiplexed biosensor (2021), Biosens. Bioelectron., 176, 112933 .

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Application

Application	Comment	Organism
analysis	construction of multiplexed direct electron transfer-type lactate and glucose sensors using a fusion enzyme between L-lactate oxidase from Aerococcus viridans, A96L/N212K mutant, which is minimized in its oxidase activity and b-type cytochrome protein. The sensors achieve simultaneous detection of lactate and glucose without cross-talking error, with the detected linear ranges of 0.5-20 mM for lactate and 0.1-5 mM for glucose, sensitivities of 4.1 nA/mM x mm2 for lactate and 56 nA/mM x mm2 for glucose, and limit of detections of 0.41 mM for lactate and 0.057 mM for glucose	Aerococcus viridans

Protein Variants

Protein Variants	Comment	Organism
A96L/N212K	mutation increases Km value and eliminates substrate inhibition	Aerococcus viridans

Organism

Organism	UniProt	Comment	Textmining
Aerococcus viridans	Q44467	-	-

Synonyms

Synonyms	Comment	Organism
LOX	-	Aerococcus viridans

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Release 2023.1 (January 2023)