Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isopropyl-beta-D-thioglucopyranoside | - |
Escherichia coli K-12 |
Cloned (Comment) | Organism |
---|---|
gene wcaG, overexpression in strain BL21(DE3) together with GDP-D-mannose-4,6-dehydratase | Escherichia coli K-12 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | effective GDP-L-fucose production in recombinant Escherichia coli by expression of GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxymannose 3,5-epimerase 4-reductase, method optimization at 25°C and 0.1 mM isopropyl-beta-D-thioglucopyranoside, overview | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + NADPH | Escherichia coli K-12 | the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview | GDP-L-fucose + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
gene wcaG | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-D-mannose + NADPH | - |
Escherichia coli K-12 | GDP-L-fucose + NADP+ | - |
r | |
GDP-4-dehydro-6-deoxy-D-mannose + NADPH | the enzyme is crucial in the biosynthesis of GDP-L-fucose together with GDP-D-mannose-4,6-dehydratase, overview | Escherichia coli K-12 | GDP-L-fucose + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase | - |
Escherichia coli K-12 |
wcaG | - |
Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli K-12 | |
NADPH | dependent on | Escherichia coli K-12 |