Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | 2.1fold activation | Mortierella alpina |
Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Mortierella alpina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Mortierella alpina | |
Cu2+ | complete inhibition at 5 mM | Mortierella alpina | |
Fe2+ | - |
Mortierella alpina | |
Mn2+ | - |
Mortierella alpina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetiic modeling | Mortierella alpina | |
1.025 | - |
NADPH | recombinant His-tagged enzyme, pH 8.0, 37°C | Mortierella alpina | |
1.047 | - |
GDP-4-dehydro-6-deoxy-alpha-D-mannose | recombinant His-tagged enzyme, pH 8.0, 37°C | Mortierella alpina | |
1.157 | - |
NADH | recombinant His-tagged enzyme, pH 8.0, 37°C | Mortierella alpina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation at 5 mM | Mortierella alpina | |
Mg2+ | slight activation at 5 mM | Mortierella alpina | |
additional information | no requirement of divalent cations | Mortierella alpina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Mortierella alpina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ | Mortierella alpina | - |
GDP-beta-L-fucose + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mortierella alpina | D3JU54 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography | Mortierella alpina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADH + H+ | the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 | Mortierella alpina | GDP-beta-L-fucose + NAD+ | product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy | ? | |
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ | - |
Mortierella alpina | GDP-beta-L-fucose + NADP+ | - |
? | |
GDP-4-dehydro-6-deoxy-alpha-D-mannose + NADPH + H+ | the enzyme synthesizes GDP-L-fucose from its substrate GDP-4-keto-6-deoxy-D-mannose. The reaction involves epimerization at both C-3 and C-5 followed by an NADPH-dependent reduction of the carbonyl at C-4 | Mortierella alpina | GDP-beta-L-fucose + NADP+ | product identification by capillary electrophoresis, electro-spray ionization-mass spectrometry, and nuclear magnetic resonance spectroscopy | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant His-tagged enzyme, SDS-PAGE | Mortierella alpina |
Synonyms | Comment | Organism |
---|---|---|
GDP-keto-6-deoxymannose 3,5-epimerase/4-reductase | - |
Mortierella alpina |
GMER | - |
Mortierella alpina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
recombinant His-tagged enzyme | Mortierella alpina |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | activity range | Mortierella alpina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant His-tagged enzyme | Mortierella alpina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | activity range | Mortierella alpina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | both NADH and NADPH may be used as the cofactor by the enzyme, which shows similar affinities to NADH and NADPH, but the Vmax value is higher for NADPH | Mortierella alpina | |
NADH | - |
Mortierella alpina | |
NADPH | - |
Mortierella alpina |