Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.271 extracted from

  • Li, L.; Kim, S.; Heo, J.; Kim, T.; Seo, J.; Han, N.
    One-pot synthesis of GDP-L-fucose by a four-enzyme cascade expressed in Lactococcus lactis (2017), J. Biotechnol., 264, 1-7 .
    View publication on PubMed

Application

Application Comment Organism
synthesis four genes (manB, manC, gmd, and wcaG) cloned from Escherichia coli are expressed in Lactococcus lactis. The one-pot reaction of ManB, ManC, Gmd, and WcaG with mannose-6-P results in the successful production of GDP-L-fucose Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
four genes (manB, manC, gmd, and wcaG) cloned from Escherichia coli are expressed in Lactococcus lactis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+ Escherichia coli
-
GDP-beta-L-fucose + NADP+
-
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+ Escherichia coli K12
-
GDP-beta-L-fucose + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P32055
-
-
Escherichia coli K12 P32055
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
-
Escherichia coli GDP-beta-L-fucose + NADP+
-
?
GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
-
Escherichia coli K12 GDP-beta-L-fucose + NADP+
-
?

Synonyms

Synonyms Comment Organism
GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase
-
Escherichia coli
wcaG
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Escherichia coli