BRENDA - Enzyme Database
show all sequences of 1.1.1.276

Cloning and sequencing of the serine dehydrogenase gene from Agrobacterium tumefaciens

Fujisawa, H.; Nagata, S.; Chowdhury, E.K.; Matsumoto, M.; Misono, H.; Biosci. Biotechnol. Biochem. 66, 1137-1139 (2002)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
DNA sequence determination and analysis, expression in Escherichia coli JM109
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-serine + NADP+
Escherichia coli
-
2-aminomalonate semialdehyde + NADPH
-
-
?
L-serine + NADP+
Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
Agrobacterium tumefaciens
?
L-serine + NADP+
Agrobacterium tumefaciens ICR 1600
-
2-aminomalonate semialdehyde + NADPH
-
Agrobacterium tumefaciens ICR 1600
?
L-serine + NADP+
Escherichia coli JM109
-
2-aminomalonate semialdehyde + NADPH
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens ICR 1600
-
-
-
Escherichia coli
-
-
-
Escherichia coli JM109
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant from Escherichia coli, to homogeneity
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0013
-
-
Escherichia coli
0.043
-
recombinant enzyme in Escherichia coli
Agrobacterium tumefaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-serine + NADP+
-
639083
Escherichia coli
2-aminomalonate semialdehyde + NADPH
-
-
-
?
L-serine + NADP+
-
639083
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
639083
Agrobacterium tumefaciens
?
L-serine + NADP+
enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism
639083
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde
639083
Agrobacterium tumefaciens
?
L-serine + NADP+
-
639083
Agrobacterium tumefaciens ICR 1600
2-aminomalonate semialdehyde + NADPH
-
639083
Agrobacterium tumefaciens ICR 1600
?
L-serine + NADP+
enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism
639083
Agrobacterium tumefaciens ICR 1600
2-aminomalonate semialdehyde + NADPH
2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde
639083
Agrobacterium tumefaciens ICR 1600
?
L-serine + NADP+
-
639083
Escherichia coli JM109
2-aminomalonate semialdehyde + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
-
Escherichia coli
tetramer
x * 25000-26600, recombinant enzyme, SDS-PAGE and amino acid sequence determination
Agrobacterium tumefaciens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
30
-
assay at
Agrobacterium tumefaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Escherichia coli
NADP+
dependent on
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA sequence determination and analysis, expression in Escherichia coli JM109
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Escherichia coli
NADP+
dependent on
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-serine + NADP+
Escherichia coli
-
2-aminomalonate semialdehyde + NADPH
-
-
?
L-serine + NADP+
Agrobacterium tumefaciens
-
2-aminomalonate semialdehyde + NADPH
-
Agrobacterium tumefaciens
?
L-serine + NADP+
Agrobacterium tumefaciens ICR 1600
-
2-aminomalonate semialdehyde + NADPH
-
Agrobacterium tumefaciens ICR 1600
?
L-serine + NADP+
Escherichia coli JM109
-
2-aminomalonate semialdehyde + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant from Escherichia coli, to homogeneity
Agrobacterium tumefaciens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0013
-
-
Escherichia coli
0.043
-
recombinant enzyme in Escherichia coli
Agrobacterium tumefaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-serine + NADP+
-
639083
Escherichia coli
2-aminomalonate semialdehyde + NADPH
-
-
-
?
L-serine + NADP+
-
639083
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
-
639083
Agrobacterium tumefaciens
?
L-serine + NADP+
enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism
639083
Agrobacterium tumefaciens
2-aminomalonate semialdehyde + NADPH
2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde
639083
Agrobacterium tumefaciens
?
L-serine + NADP+
-
639083
Agrobacterium tumefaciens ICR 1600
2-aminomalonate semialdehyde + NADPH
-
639083
Agrobacterium tumefaciens ICR 1600
?
L-serine + NADP+
enzyme belongs to the short-chain alcohol dehydrogenase family, similar reaction mechanism
639083
Agrobacterium tumefaciens ICR 1600
2-aminomalonate semialdehyde + NADPH
2-aminomalonate semialdehyde spontaneously decarboxylates into 2-aminoacetaldehyde
639083
Agrobacterium tumefaciens ICR 1600
?
L-serine + NADP+
-
639083
Escherichia coli JM109
2-aminomalonate semialdehyde + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Escherichia coli
tetramer
x * 25000-26600, recombinant enzyme, SDS-PAGE and amino acid sequence determination
Agrobacterium tumefaciens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Escherichia coli
30
-
assay at
Agrobacterium tumefaciens
Other publictions for EC 1.1.1.276
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
639084
Fujisawa
Characterization of short-chai ...
Saccharomyces cerevisiae, Escherichia coli, Saccharomyces cerevisiae YMR226C, Escherichia coli YdfG
Biochim. Biophys. Acta
1645
89-94
2003
-
-
2
-
-
-
-
8
-
-
4
4
-
4
-
-
2
-
-
-
2
-
40
2
-
-
-
2
6
2
-
2
4
-
-
-
-
-
2
4
-
-
-
-
-
-
8
-
-
4
4
-
-
-
2
-
-
2
-
40
2
-
-
2
6
2
-
2
-
-
-
-
-
-
-
639083
Fujisawa
Cloning and sequencing of the ...
Agrobacterium tumefaciens, Escherichia coli, Agrobacterium tumefaciens ICR 1600, Escherichia coli JM109
Biosci. Biotechnol. Biochem.
66
1137-1139
2002
-
-
1
-
-
-
-
-
-
-
-
4
-
34
-
-
1
-
-
-
2
-
6
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
2
-
6
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
639082
Chowdhury
A novel NADP+ dependent serine ...
Agrobacterium tumefaciens
Biosci. Biotechnol. Biochem.
61
152-157
1997
-
-
-
-
-
-
10
5
-
1
1
1
-
1
-
-
1
1
-
-
1
-
10
-
-
1
-
1
-
1
-
1
2
10
-
-
-
-
-
2
-
-
-
-
10
10
5
-
1
1
1
-
-
-
1
-
-
1
-
10
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-