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Literature summary for 1.1.1.28 extracted from
- Enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus (2015), Biotechnol. Lett., 37, 1233-1241.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ldhD, subcloning in Escherichia coli straiin DH5alpha, functional overexpression of phenyllactic acid-overproducing enzyme mutant in Escherichia coli strain BL21 (DE3), optimal induction at 25°C | Lactiplantibacillus pentosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y52A | site-directed mutagenesis, enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus | Lactiplantibacillus pentosus |
| Y52L | site-directed mutagenesis, enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus | Lactiplantibacillus pentosus |
| Y52V | site-directed mutagenesis, enhancement of phenyllactic acid biosynthesis by recognition site replacement of D-lactate dehydrogenase from Lactobacillus pentosus. Escherichia coli pET-28a-d-ldh produces 12.2 g phenyllactate/l in 3 h with a molar conversion rate of 61%, while Escherichia coli pET-28a-d-ldhY52V produces 15.6 g phenyllactate/l with a molar conversion rate of 77%. Site-directed mutagenesis of d-ldh markedly improves D-phenyllactate yield and substrate conversion rate. Phenyllactate yield does not show a significant increase when higher glucose amounts are added. Method optimization, overview | Lactiplantibacillus pentosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-3-phenylpyruvate + NADH + H+ | Lactiplantibacillus pentosus | - |
D-3-phenyllactate + NAD+ | i.e. 2-hydroxy-3-phenylpropanoic acid | r |  |
| pyruvate + NADH + H+ | Lactiplantibacillus pentosus | - |
(R)-lactate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus pentosus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-3-phenylpyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | D-3-phenyllactate + NAD+ | i.e. 2-hydroxy-3-phenylpropanoic acid | r | |
| additional information | D-LDH is an NADH-dependent reductase, substrate reduction is accompanied by the oxidation of NADH to NAD? | Lactiplantibacillus pentosus | ? | - |
? | |
| pyruvate + NADH + H+ | - |
Lactiplantibacillus pentosus | (R)-lactate + NAD+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-LDH | - |
Lactiplantibacillus pentosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Lactiplantibacillus pentosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Lactiplantibacillus pentosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Lactiplantibacillus pentosus | |
| NADH | - |
Lactiplantibacillus pentosus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the side chain of residue Tyr52 locates very near the C3 substituent group of 2-ketoacid substrate and is associated with the recognition of substrate side chain | Lactiplantibacillus pentosus |
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Release 2023.1 (January 2023)
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