Application | Comment | Organism |
---|---|---|
synthesis | asymmetric synthesis of (R)-2-hydroxy-4-phenylbutyric acid using recombinant Pichia pastoris expressing the Tyr52Leu variant of D-lactate dehydrogenase from Lactobacillus plantarum. The recombinant yeast cells show catalytic activity at a high concentration of 2-oxo-4-phenylbutyric acid (380 mM, 76 g/l). Under optimized reaction conditions (pH 7.5, 37°C, and 2% glucose), a full conversion with over 95% reaction yield and about 100% product enantiomeric excess is achieved | Lactiplantibacillus plantarum |
Protein Variants | Comment | Organism |
---|---|---|
Y52L | mutant used for synthesis of (R)-2-hydroxy-4-phenylbutyric acid in Pichia pastoris | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-phenylbutyric acid + reduced acceptor | - |
Lactiplantibacillus plantarum | (R)-2-hydroxy-4-phenylbutyric acid + acceptor | - |
? |