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Literature summary for 1.1.1.28 extracted from

  • Wang, Y.; Bai, Y.; Fan, T.; Zheng, X.; Cai, Y.
    Reducing 3,4-dihydroxyphenylpyruvic acid to D-3,4-dihydroxyphenyllactic acid via a coenzyme nonspecific D-lactate dehydrogenase from Lactobacillus reuteri (2018), J. Appl. Microbiol., 125, 1739-1748 .
    View publication on PubMed
Search for more literature for 1.1.1.28

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, 21.9% residual activity Limosilactobacillus reuteri
additional information neither inhibitory nor stimulating: Ca2+, K+, Na+, Mn2+, Zn2+, Mg2+, Fe2+, Ni2+ and Co2+ and EDTA Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 3,4-dihydroxyphenylpyruvic acid cosubstrate NADH, pH 8.0, 25°C Limosilactobacillus reuteri
0.1
-
 3,4-dihydroxyphenylpyruvic acid cosubstrate NADPH, pH 8.0, 25°C Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
additional information neither inhibitory nor stimulating: Ca2+, K+, Na+, Mn2+, Zn2+, Mg2+, Fe2+, Ni2+ and Co2+ Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
-
-
-
Limosilactobacillus reuteri JN516
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83.2
-
 pH 8.0, 25°C Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADPH + H+
-
 Limosilactobacillus reuteri (R)-2-hydroxyglutarate + NADP+
-
 ?
2-oxoglutarate + NADPH + H+
-
 Limosilactobacillus reuteri JN516 (R)-2-hydroxyglutarate + NADP+
-
 ?
3,4-dihydroxyphenylpyruvic acid + NADH + H+
-
 Limosilactobacillus reuteri D-3,4-dihydroxyphenyllactic acid + NAD+
-
 ?
3,4-dihydroxyphenylpyruvic acid + NADH + H+
-
 Limosilactobacillus reuteri JN516 D-3,4-dihydroxyphenyllactic acid + NAD+
-
 ?
3-methyl-2-oxobutanoate + NADH + H+
-
 Limosilactobacillus reuteri (R)-2-hydroxy-3-methylbutanoate + NAD+
-
 ?
3-methyl-2-oxobutanoate + NADH + H+
-
 Limosilactobacillus reuteri JN516 (R)-2-hydroxy-3-methylbutanoate + NAD+
-
 ?
4-methyl-2-oxopentanoate + NADH + H+
-
 Limosilactobacillus reuteri (R)-2-hydroxy-4-methylpentanoate + NAD+
-
 ?
4-methyl-2-oxopentanoate + NADH + H+
-
 Limosilactobacillus reuteri JN516 (R)-2-hydroxy-4-methylpentanoate + NAD+
-
 ?
benzoylformate + NADH + H+
-
 Limosilactobacillus reuteri phenylglyoxylate + NAD+
-
 ?
additional information no or poor substrates: 2-oxobutanoate, phenylpyruvate, 4-hydroxyphenylpyruvate Limosilactobacillus reuteri ?
-
-
additional information no or poor substrates: 2-oxobutanoate, phenylpyruvate, 4-hydroxyphenylpyruvate Limosilactobacillus reuteri JN516 ?
-
-

Synonyms

Synonyms Comment Organism
D-LDH82319
-
 Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Limosilactobacillus reuteri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 3,4-dihydroxyphenylpyruvic acid cosubstrate NADPH, pH 8.0, 25°C Limosilactobacillus reuteri
2.17
-
 3,4-dihydroxyphenylpyruvic acid cosubstrate NADH, pH 8.0, 25°C Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Limosilactobacillus reuteri

pH Range

pH Minimum pH Maximum Comment Organism
3 4 no activity observed Limosilactobacillus reuteri

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 1 h, more than 80% of maximum activity Limosilactobacillus reuteri

Cofactor

Cofactor Comment Organism Structure
NADH NADH is preferred over NADPH Limosilactobacillus reuteri
NADPH NADH is preferred over NADPH Limosilactobacillus reuteri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 3,4-dihydroxyphenylpyruvic acid cosubstrate NADPH, pH 8.0, 25°C Limosilactobacillus reuteri
24.07
-
 3,4-dihydroxyphenylpyruvic acid cosubstrate NADH, pH 8.0, 25°C Limosilactobacillus reuteri