BRENDA - Enzyme Database
show all sequences of 1.1.1.282

A phylogenomic analysis of the shikimate dehydrogenases reveals broadscale functional diversification and identifies one functionally distinct subclass

Singh, S.; Stavrinides, J.; Christendat, D.; Guttman, D.S.; Mol. Biol. Evol. 25, 2221-2232 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs)
Pseudomonas putida KT2440
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.572
-
NAD+
in the presence of shikimate
Pseudomonas putida KT2440
0.783
-
quinate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
1.083
-
NAD+
in the presence of quinate
Pseudomonas putida KT2440
4.2
-
shikimate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida KT2440
Q88GF6
-
-
Pseudomonas putida KT2440
Q88JP1
-
-
Pseudomonas putida KT2440
Q88K85
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by nickel-nitrilotriacetic acid affinity chromatography
Pseudomonas putida KT2440
Storage Stability
Storage Stability
Organism
4°C, 10 mM Tris-HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol
Pseudomonas putida KT2440
4°C, 10 mM Tris–HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol
Pseudomonas putida KT2440
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-quinate + NAD+
-
694093
Pseudomonas putida KT2440
4-dehydroquinate + NADH + H+
-
-
-
r
additional information
broad extent to which the SDH enzyme superfamily has diversified. 5 evolutionarily distinct SDH homologs in the genome of the common soil-inhabiting bacterium, Pseudomonas putida KT2440
694093
Pseudomonas putida KT2440
?
-
-
-
?
quinate + NAD(P)+
-
694093
Pseudomonas putida KT2440
3-dehydroquinate + NAD(P)H + H+
-
-
-
?
shikimate + NAD(P)+
-
694093
Pseudomonas putida KT2440
3-dehydroshikimate + NAD(P)H + H+
-
-
-
?
shikimate + NAD+
-
694093
Pseudomonas putida KT2440
3-dehydroshikimate + NADH + H+
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
rifI
-
Pseudomonas putida KT2440
SDH
-
Pseudomonas putida KT2440
sdhL
-
Pseudomonas putida KT2440
YdiB
-
Pseudomonas putida KT2440
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
37.7
-
quinate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
55.7
-
shikimate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
55.7
-
NAD+
in the presence of quinate
Pseudomonas putida KT2440
61.1
-
NAD+
in the presence of shikimate
Pseudomonas putida KT2440
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Pseudomonas putida KT2440
Cloned(Commentary) (protein specific)
Commentary
Organism
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs)
Pseudomonas putida KT2440
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Pseudomonas putida KT2440
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.572
-
NAD+
in the presence of shikimate
Pseudomonas putida KT2440
0.783
-
quinate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
1.083
-
NAD+
in the presence of quinate
Pseudomonas putida KT2440
4.2
-
shikimate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
Purification (Commentary) (protein specific)
Commentary
Organism
by nickel-nitrilotriacetic acid affinity chromatography
Pseudomonas putida KT2440
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 10 mM Tris-HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol
Pseudomonas putida KT2440
4°C, 10 mM Tris–HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol
Pseudomonas putida KT2440
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-quinate + NAD+
-
694093
Pseudomonas putida KT2440
4-dehydroquinate + NADH + H+
-
-
-
r
additional information
broad extent to which the SDH enzyme superfamily has diversified. 5 evolutionarily distinct SDH homologs in the genome of the common soil-inhabiting bacterium, Pseudomonas putida KT2440
694093
Pseudomonas putida KT2440
?
-
-
-
?
quinate + NAD(P)+
-
694093
Pseudomonas putida KT2440
3-dehydroquinate + NAD(P)H + H+
-
-
-
?
shikimate + NAD(P)+
-
694093
Pseudomonas putida KT2440
3-dehydroshikimate + NAD(P)H + H+
-
-
-
?
shikimate + NAD+
-
694093
Pseudomonas putida KT2440
3-dehydroshikimate + NADH + H+
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
37.7
-
quinate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
55.7
-
shikimate
in the presence of 2 mM NAD+
Pseudomonas putida KT2440
55.7
-
NAD+
in the presence of quinate
Pseudomonas putida KT2440
61.1
-
NAD+
in the presence of shikimate
Pseudomonas putida KT2440
Other publictions for EC 1.1.1.282
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740881
Garcia
The role of the ydiB gene, whi ...
Escherichia coli, Escherichia coli JM101
J. Mol. Microbiol. Biotechnol.
27
11-21
2016
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1
1
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1
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6
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12
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2
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1
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6
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6
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739956
Peek
The shikimate dehydrogenase fa ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Arch. Biochem. Biophys.
566
85-99
2015
-
2
2
2
-
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5
-
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6
-
4
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4
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9
2
2
2
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4
2
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4
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2
2
4
2
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5
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6
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9
2
2
-
-
4
2
-
-
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-
8
8
-
-
-
740724
Guo
Molecular characterization of ...
Populus trichocarpa, Populus trichocarpa Nisqually-1
J. Biol. Chem.
289
23846-23858
2014
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1
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5
-
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3
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5
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1
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7
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3
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5
1
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1
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2
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1
2
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5
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3
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1
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7
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3
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1
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1
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4
4
-
-
-
724089
Kubota
Characterization of shikimate ...
Corynebacterium glutamicum
Appl. Microbiol. Biotechnol.
97
8139-8149
2013
-
-
1
-
1
-
-
6
-
-
2
3
-
1
-
-
1
-
-
-
-
-
5
1
3
-
-
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5
2
1
-
3
-
-
-
-
-
1
3
-
1
-
-
-
-
6
-
-
2
3
-
-
-
1
-
-
-
-
5
1
-
-
-
5
2
1
-
-
1
1
1
1
5
5
727115
Höppner
Enzyme-substrate complexes of ...
Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032
Biol. Chem.
394
1505-1516
2013
-
-
-
1
-
-
-
8
-
-
-
5
-
7
-
-
-
-
-
-
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5
1
4
1
-
-
8
2
-
-
3
-
-
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-
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3
1
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8
-
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5
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5
1
1
-
-
8
2
-
-
-
-
1
1
-
8
8
740100
Peek
Insights into the function of ...
Pseudomonas putida, Pseudomonas putida KT 2240
Biochim. Biophys. Acta
1834
516-523
2013
-
-
1
1
4
-
-
6
-
1
-
4
-
5
-
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1
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6
1
2
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6
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3
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1
3
1
4
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6
-
1
-
4
-
-
-
1
-
-
-
-
6
1
-
-
-
6
-
-
-
-
-
1
1
-
6
6
694093
Singh
A phylogenomic analysis of the ...
Pseudomonas putida KT2440
Mol. Biol. Evol.
25
2221-2232
2008
-
-
3
-
-
-
-
4
-
-
-
-
-
3
-
-
3
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-
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-
3
11
-
6
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-
-
4
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-
3
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-
3
3
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-
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-
4
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-
-
3
-
-
-
3
11
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
667700
Singh
Structure of Arabidopsis dehyd ...
Arabidopsis thaliana
Biochemistry
45
10406
2006
-
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-
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1
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1
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1
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1
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656292
Lindner
Site-directed mutagenesis of t ...
Escherichia coli
J. Biol. Chem.
280
7162-7169
2005
-
2
-
-
8
-
-
30
-
-
3
-
-
3
-
-
-
-
-
-
-
-
4
1
2
-
-
-
30
-
-
-
4
-
-
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-
2
-
4
-
8
-
-
-
-
30
-
-
3
-
-
-
-
-
-
-
-
-
4
1
-
-
-
30
-
-
-
-
-
-
-
-
-
-
669328
Singh
Crystal structure of a novel s ...
Enterococcus faecalis, Enterococcus faecium, Lactiplantibacillus plantarum, Listeria monocytogenes, Salmonella enterica subsp. enterica serovar Typhimurium, Shigella flexneri, Streptococcus pyogenes, Escherichia coli, Haemophilus influenzae
J. Biol. Chem.
280
17101-17108
2005
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-
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9
-
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9
-
18
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8
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8
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9
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644536
Michel
Structures of shikimate dehydr ...
Escherichia coli
J. Biol. Chem.
278
19463-19472
2003
-
-
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1
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8
-
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1
1
-
4
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1
2
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-
1
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4
1
-
1
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6
1
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-
2
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2
1
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8
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1
1
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1
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1
-
4
1
1
-
-
6
1
-
-
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-
-
-
-
-
-
644537
Benach
The 2.3-Å crystal structure o ...
Escherichia coli
J. Biol. Chem.
278
19176-19182
2003
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1
1
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1
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1
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1
2
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3
1
1
1
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1
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4
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1
1
4
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1
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1
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3
1
1
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1
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657079
Ossipov
-
Broad-specificity quinate (shi ...
Pinus taeda
Plant Physiol. Biochem.
38
923-928
2000
-
-
-
-
-
-
2
7
-
-
2
1
-
1
-
-
1
-
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1
1
-
3
-
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1
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2
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2
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2
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2
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7
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2
1
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1
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1
1
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3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
654384
Bruce
-
Hydroaromatic metabolism in Rh ...
Rhodococcus rhodochrous, Rhodococcus rhodochrous N75
Arch. Microbiol.
154
179-186
1990
-
-
-
-
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8
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2
2
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5
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1
2
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1
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17
1
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1
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2
1
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7
-
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7
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8
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2
2
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1
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1
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17
1
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1
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2
1
-
-
-
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654912
Osipov
-
The role of quinate dehydrogen ...
Pinus sylvestris, Larix sibirica
Biokhimiya
51
230-236
1986
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2
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2
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2
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3
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286356
Barea
Purification and characterizat ...
Neurospora crassa
Biochim. Biophys. Acta
524
1-14
1978
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2
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1
1
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1
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1
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1
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1
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1
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