Cloned (Comment) | Organism |
---|---|
gene ADH3, quantitative PCR enzyme expression analysis | Lactuca sativa |
quantitative PCR enzyme expression analysis | Lactuca serriola |
quantitative PCR enzyme expression analysis | Lactuca saligna |
quantitative PCR enzyme expression analysis | Lactuca virosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NADH + H+ | Lactuca serriola | - |
GSSG + ammonia + NAD+ | - |
ir | |
S-nitrosoglutathione + NADH + H+ | Lactuca sativa | - |
GSSG + ammonia + NAD+ | - |
ir | |
S-nitrosoglutathione + NADH + H+ | Lactuca saligna | - |
GSSG + ammonia + NAD+ | - |
ir | |
S-nitrosoglutathione + NADH + H+ | Lactuca virosa | - |
GSSG + ammonia + NAD+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactuca saligna | A0A6S7LX43 | cv. CGN 05271 | - |
Lactuca sativa | J7GHV7 | cvs. UCDM2 and Mariska | - |
Lactuca serriola | - |
cv. LSE/18 | - |
Lactuca virosa | - |
cv. NVRS 10.001 602 | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially from leaf extract by gel filtration | Lactuca serriola |
native enzyme partially from leaf extract by gel filtration | Lactuca sativa |
native enzyme partially from leaf extract by gel filtration | Lactuca saligna |
native enzyme partially from leaf extract by gel filtration | Lactuca virosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | GSNOR and GSNO immunodetection by confocal laser scanning microscopy | Lactuca serriola | - |
leaf | GSNOR and GSNO immunodetection by confocal laser scanning microscopy | Lactuca sativa | - |
leaf | GSNOR and GSNO immunodetection by confocal laser scanning microscopy | Lactuca saligna | - |
leaf | GSNOR and GSNO immunodetection by confocal laser scanning microscopy | Lactuca virosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-nitrosoglutathione + NADH + H+ | - |
Lactuca serriola | GSSG + ammonia + NAD+ | - |
ir | |
S-nitrosoglutathione + NADH + H+ | - |
Lactuca sativa | GSSG + ammonia + NAD+ | - |
ir | |
S-nitrosoglutathione + NADH + H+ | - |
Lactuca saligna | GSSG + ammonia + NAD+ | - |
ir | |
S-nitrosoglutathione + NADH + H+ | - |
Lactuca virosa | GSSG + ammonia + NAD+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
ADH3 | - |
Lactuca sativa |
GSNOR | - |
Lactuca serriola |
GSNOR | - |
Lactuca sativa |
GSNOR | - |
Lactuca saligna |
GSNOR | - |
Lactuca virosa |
S-nitrosoglutathione reductase | - |
Lactuca serriola |
S-nitrosoglutathione reductase | - |
Lactuca sativa |
S-nitrosoglutathione reductase | - |
Lactuca saligna |
S-nitrosoglutathione reductase | - |
Lactuca virosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Lactuca serriola |
25 | - |
assay at | Lactuca sativa |
25 | - |
assay at | Lactuca saligna |
25 | - |
assay at | Lactuca virosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Lactuca serriola |
8 | - |
assay at | Lactuca sativa |
8 | - |
assay at | Lactuca saligna |
8 | - |
assay at | Lactuca virosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Lactuca serriola | |
NADH | - |
Lactuca sativa | |
NADH | - |
Lactuca saligna | |
NADH | - |
Lactuca virosa |
Organism | Comment | Expression |
---|---|---|
Lactuca serriola | GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview | up |
Lactuca sativa | GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview | up |
Lactuca saligna | GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview | up |
Lactuca virosa | GSNOR expression, level and activity are studied in leaves of selected genotypes of lettuce (Lactuca sativa) and wild Lactuca spp. during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi). GSNOR expression is increased in all genotypes both in the early phase at 6 hpi and later phase at 72 hpi, with a high increase observed in Lactuca sativa UCDM2 responses to all three pathogens. GSNOR protein also shows two-phase increase, with highest changes in Lactuca virosa-Bremia lactucae and Lactuca sativa cv. UCDM2-Golovinomyces cichoracearum pathosystems, whereas Pseudoidium neolycopersici induces GSNOR protein at 72 hpi in all genotypes. Similarly, a general pattern of modulated GSNOR activities in response to biotrophic mildews involves a two-phase increase at 6 and 72 hpi. Lettuce downy mildew infection causes GSNOR activity slightly increased only in resistant Lactuca saligna and Lactuca virosa genotypes. But all genotypes show increased GSNOR activity both at 6 and 72 hpi by lettuce powdery mildew. GSNOR-mediated decrease of S-nitrosothiols is observed as a general feature of Lactuca spp. response to mildew infection, which is also confirmed by immunohistochemical detection of GSNOR and GSNO in infected plant tissues. GSNOR is differentially modulated in interactions of susceptible and resistant Lactuca spp. genotypes with fungal mildews and uncover the role of S-nitrosylation in molecular mechanisms of plant responses to biotrophic pathogens. S-Nitrosothiol profiles during pathogenesis and expression pattern, overview | up |
General Information | Comment | Organism |
---|---|---|
evolution | GSNOR is a member of class III alcohol dehydrogenase family | Lactuca serriola |
evolution | GSNOR is a member of class III alcohol dehydrogenase family | Lactuca sativa |
evolution | GSNOR is a member of class III alcohol dehydrogenase family | Lactuca saligna |
evolution | GSNOR is a member of class III alcohol dehydrogenase family | Lactuca virosa |
malfunction | in leaves of Lactuca saligna during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols | Lactuca saligna |
malfunction | in leaves of Lactuca sativa during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is highly increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols | Lactuca sativa |
malfunction | in leaves of Lactuca serriola during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols | Lactuca serriola |
malfunction | in leaves of Lactuca virosa during interactions with biotrophic mildews, Bremia lactucae (lettuce downy mildew), Golovinomyces cichoracearum (lettuce powdery mildew) and non-pathogen Pseudoidium neolycopersici (tomato powdery mildew) during 168 h post inoculation (hpi), the GSNOR expression is increased both in the early phase at 6 hpi and later phase at 72 hpi, with increased GSNOR-mediated decrease of S-nitrosothiols | Lactuca virosa |
physiological function | S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) | Lactuca serriola |
physiological function | S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) | Lactuca sativa |
physiological function | S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) | Lactuca saligna |
physiological function | S-nitrosylation belongs to principal signalling pathways of nitric oxide in plant development and stress responses. Protein S-nitrosylation is regulated by S-nitrosoglutathione reductase (GSNOR) as a key catabolic enzyme of S-nitrosoglutathione (GSNO), the major intracellular S-nitrosothiol. GSNOR metabolizes S-nitrosoglutathione irreversibly to glutathione disulphide (GSSG) and ammonia (NH3) | Lactuca virosa |