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Literature summary for 1.1.1.290 extracted from

  • Ha, J.Y.; Lee, J.H.; Kim, K.H.; Kim, D.J.; Lee, H.H.; Kim, H.K.; Yoon, H.J.; Suh, S.W.
    Overexpression, crystallization and preliminary X-ray crystallographic analysis of erythronate-4-phosphate dehydrogenase from Pseudomonas aeruginosa (2006), Acta Crystallogr. Sect. F, 62, 139-141.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion at 24°C K using 0.7 M ammonium dihydrogen phosphate, 0.4 M ammonium tartrate, 0.1 M sodium citrate pH 5.6 and 10 mM cupric chloride Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 estimated by dynamic light-scattering analysis Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal-chelate chromatography on Ni-NTA resin and size exclusion chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
erythronate-4-phosphate + NAD+
-
 Pseudomonas aeruginosa 3-hydroxy-4-phospho-hydroxy-alpha-ketobutyrate + NADH
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 42 067, enzyme including a C-terminal tag, enzyme consists of two identical 380-residue subunits Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
erythronate-4-phosphate dehydrogenase
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas aeruginosa