Literature summary for 1.1.1.291 extracted from

Alhapel, A.; Darley, D.J.; Wagener, N.; Eckel, E.; Elsner, N.; Pierik, A.J.
Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri (2006), Proc. Natl. Acad. Sci. USA, 103, 12341-12346.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Eubacterium barkeri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	NAD+	-	Eubacterium barkeri
1.1	-	2-hydroxymethylglutarate	-	Eubacterium barkeri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	4 * 32000, SDS-PAGE	Eubacterium barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-formylglutarate + NADH + H+	Eubacterium barkeri	the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri	2-hydroxymethylglutarate + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Eubacterium barkeri	-	-	-

Oxidation Stability

Oxidation Stability	Organism
instability under anaerobic conditions	Eubacterium barkeri

Purification (Commentary)

Purification (Comment)	Organism
-	Eubacterium barkeri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-formylglutarate + NADH + H+	the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri	Eubacterium barkeri	2-hydroxymethylglutarate + NAD+	-	?
2-hydroxymethylglutarate + NAD+	-	Eubacterium barkeri	2-formylglutarate + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 32000, SDS-PAGE	Eubacterium barkeri

Synonyms

Synonyms	Comment	Organism
HgD	-	Eubacterium barkeri

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	no activity with NADP+	Eubacterium barkeri
NADH	-	Eubacterium barkeri

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Release 2023.1 (January 2023)