Cloned (Comment) | Organism |
---|---|
gene NYC1, real-time PCR enzyme expression analysis | Brassica oleracea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | analysis of expression of a gene encoding a putative CBR (BoNYC1) during postharvest senescence of broccoli and of effects of postharvest physical treatements, overview | Brassica oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in broccoli, treatments with UV-C can delay Chl degradation, diminish respiration rates and reduce the loss of sugars and proteins during postharvest storage | Brassica oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Brassica oleracea | 9507 | - |
thylakoid membrane | - |
Brassica oleracea | 42651 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea | - |
italica group, cv. Avenger | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
branchlet | - |
Brassica oleracea | - |
floret | - |
Brassica oleracea | - |
additional information | BoNYC1 expression increases during first days of postharvest, but decreases in advanced senescence stages, simultaneously with chlorophyll degradation. Treatments with cytokinins and 1-MCP delays the increment of BoNYC1 expression whereas ethylene accelerates the process | Brassica oleracea | - |
stem | - |
Brassica oleracea | - |
Synonyms | Comment | Organism |
---|---|---|
BoNYC1 | - |
Brassica oleracea |
CBR | - |
Brassica oleracea |
chlorophyll b reductase | - |
Brassica oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
in vitro assay at, storage temperature of broccoli | Brassica oleracea |
Organism | Comment | Expression |
---|---|---|
Brassica oleracea | cytokinins and 1-MCP inhibit developmental increase in BoNYC1 expression. Samples treated with heat show a significant lower relative expression of BoNYC1 in comparison to controls after 72 h but not after 120 h of storage | down |
Brassica oleracea | ethylene, via 2-chloroethylphosphonic acid (ethephon, an ethylene-releasing agent), enhances developmental increase in BoNYC1 expression. Samples treated with visible light show a higher increment of BoNYC1 expression in relation to controls after 72 h but the expression is similar to those of controls after 120 h. Expressions of NYC1 and its homologue, NOL, increase markedly under intense light conditions | up |
General Information | Comment | Organism |
---|---|---|
malfunction | in broccoli, treatments with UV-C can delay Chl degradation, diminish respiration rates and reduce the loss of sugars and proteins during postharvest storage | Brassica oleracea |
metabolism | during senescence, chlorophylls are degraded with the purpose of avoiding presence of photoactive molecules. Chlorophyll b must be previously converted to chlorophyll a in order to be catabolized. This reduction process is catalyzed by two enzymes, chlorophyll b reductase (CBR) and hydroxymethyl chlorophyll a reductase (HCAR) | Brassica oleracea |
physiological function | chlorophyll molecules (Chl a and Chl b) are located in the thylakoid membranes inside the chloroplasts forming part of the light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII). During senescence, thylakoid membranes are dismantled and chlorophyll molecules are released and catabolized. Chlorophyll degradation is under strict control because free chlorophyll molecules or their intermediates are photoactive and can generate highly reactive toxic radicals. During senescence, chlorophylls are degraded, therefore chlorophyll b must be previously converted to chlorophyll a in order to be catabolized. This reduction process is catalyzed by two enzymes, chlorophyll b reductase (CBR) and hydroxymethyl chlorophyll a reductase (HCAR). Chlorophyll b degradation is required for the degradation of light-harvesting complex II and thylakoid grana during leaf senescence | Brassica oleracea |