Any feedback?
Literature summary for 1.1.1.3 extracted from
- Isolation and characterization of two homoserine dehydrogenases from maize suspension cultures (1979), J. Biol. Chem., 254, 1349-1355.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-threonine | - |
Zea mays |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
NADPH | threonine resistant isozyme | Zea mays | |
| 0.036 | - |
NADPH | threonine-resistant enzyme, pH and temperature not specified in the publication | Zea mays | |
| 0.04 | - |
NADPH | threonine sensitive isozyme | Zea mays | |
| 0.04 | - |
NADPH | threonine-sensitive enzyme, pH and temperature not specified in the publication | Zea mays | |
| 0.15 | - |
L-aspartate 4-semialdehyde | threonine resistant isozyme | Zea mays | |
| 0.15 | - |
L-aspartate 4-semialdehyde | threonine-resistant enzyme, pH and temperature not specified in the publication | Zea mays | |
| 0.25 | - |
L-aspartate 4-semialdehyde | threonine sensitive isozyme | Zea mays | |
| 0.25 | - |
L-aspartate 4-semialdehyde | threonine-sensitive enzyme, pH and temperature not specified in the publication | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE, threonine resistant isozyme | Zea mays |
| 70000 | - |
gel filtration, threonine insensitive isozyme | Zea mays |
| 89000 | - |
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme | Zea mays |
| 93000 | - |
x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme | Zea mays |
| 190000 | - |
gel filtration threonine sensitive isozyme | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
corn | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| threonine sensitive and threonine insensitive isozymes | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 343 | - |
- |
Zea mays |
| 360 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 30 mM potassium phosphate buffer, pH 7.5, 50% v/v glycerol, threonine resistant isozyme at least 2 months stable, threonine sensitive isozyme at least 4 months stable | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate 4-semialdehyde + NADPH + H+ | - |
Zea mays | L-homoserine + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE, threonine resistant isozyme | Zea mays |
| dimer | x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme | Zea mays |
| tetramer | x * 89000 + x * 93000, SDS-PAGE, threonine sensitive isozyme | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Zea mays | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
