Cloned (Comment) | Organism |
---|---|
gene hom, DNA and amino acid sequence determination and analysis, expression in the auxotroph mutant Escherichia coli CGSC 5075 | Streptomyces clavuligerus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a hom disruption mutant by insertional inactivation via double crossover leading to up to 4.3fold and 2fold increases in intracellular free L-lysine concentration and specific cephamycin C production, respectively, during stationary phase in chemically defined medium, overview | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NAD(P)+ | Streptomyces clavuligerus | - |
L-aspartate 4-semialdehyde + NAD(P)H | - |
r | |
L-homoserine + NAD(P)+ | Streptomyces clavuligerus NRRL 3585 | - |
L-aspartate 4-semialdehyde + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | Q56R01 | gene hom | - |
Streptomyces clavuligerus NRRL 3585 | Q56R01 | gene hom | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NAD(P)+ | - |
Streptomyces clavuligerus | L-aspartate 4-semialdehyde + NAD(P)H | - |
r | |
L-homoserine + NAD(P)+ | - |
Streptomyces clavuligerus NRRL 3585 | L-aspartate 4-semialdehyde + NAD(P)H | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptomyces clavuligerus | |
NADPH | - |
Streptomyces clavuligerus |