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Literature summary for 1.1.1.3 extracted from

  • Schroeder, A.C.; Zhu, C.; Yanamadala, S.R.; Cahoon, R.E.; Arkus, K.A.; Wachsstock, L.; Bleeke, J.; Krishnan, H.B.; Jez, J.M.
    Threonine-insensitive homoserine dehydrogenase from soybean: genomic organization, kinetic mechanism, and in vivo activity (2010), J. Biol. Chem., 285, 827-834.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Glycine max

Inhibitors

Inhibitors Comment Organism Structure
L-threonine
-
 Glycine max
additional information enzyme is not inhibited by other aspartate-derived amino acids than threonine Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 NADPH pH 8.0, 25°C Glycine max
0.034
-
 NADP+ pH 8.0, 25°C Glycine max
0.039
-
 NADPH pH 8.0, 25°C Glycine max
0.098
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
0.158
-
 NADH pH 8.0, 25°C Glycine max
0.19
-
 NADH pH 8.0, 25°C Glycine max
0.213
-
 NADH pH 8.0, 25°C Glycine max
0.235
-
 NADP+ pH 8.0, 25°C Glycine max
0.245
-
 NADP+ pH 8.0, 25°C Glycine max
0.275
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.569
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
0.69
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.845
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
1.08
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
1.19
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
1.25
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
2.19
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
2.7
-
 NAD+ pH 8.0, 25°C Glycine max
9.57
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
13.4
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
17.4
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
24.1
-
 NAD+ pH 8.0, 25°C Glycine max
24.9
-
 NAD+ pH 8.0, 25°C Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40600, calculated, 2 * 40000, SDS-PAGE Glycine max
40600
-
 2 * 40600, calculated, 2 * 40000, SDS-PAGE Glycine max
70000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max O63067 bifunctional aspartokinase-homoserine dehydrogenase AK-HSD-2
-
Glycine max O65027 bifunctional aspartokinase-homoserine dehydrogenase AK-HSD-1
-
Glycine max Q3S3F6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Glycine max

Reaction

Reaction Comment Organism Reaction ID
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ ordered bi bi kinetic mechanism in which nicotinamide cofactor binds first and leaves last in the reaction sequence Glycine max
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
 pH 8.0, 25°C Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + NADH
-
 Glycine max L-homoserine + NAD+
-
 r
L-aspartate 4-semialdehyde + NADPH
-
 Glycine max L-homoserine + NADP+
-
 r
L-homoserine + NAD+
-
 Glycine max L-aspartate 4-semialdehyde + NADH
-
 r
L-homoserine + NADP+
-
 Glycine max L-aspartate 4-semialdehyde + NADPH
-
 r
additional information enzyme does not show aspartate kinase activity Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40600, calculated, 2 * 40000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
AK-HSD-1
-
 Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.052
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.07
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
1.43
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
2.8
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
7
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
9.68
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
10.15
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
11.58
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
12.98
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
19.3
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
22.58
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max

Cofactor

Cofactor Comment Organism Structure
NADH GmHSD displays a 1.6fold preference for NADPH over NADH as the cofactor in the oxidation reaction. In the reduction reaction NADP+ is favored nearly 4fold as the cofactor Glycine max
NADPH GmHSD displays a 1.6fold preference for NADPH over NADH as the cofactor in the oxidation reaction. In the reduction reaction NADP+ is favored nearly 4fold as the cofactor Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
160 240 L-threonine pH 8.0, 25°C Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.039
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.082
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
0.095
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.18
-
 NADP+ pH 8.0, 25°C Glycine max
0.256
-
 L-homoserine cosubstrate NADP+, pH 8.0, 25°C Glycine max
0.268
-
 NADP+ pH 8.0, 25°C Glycine max
0.281
-
 NAD+ pH 8.0, 25°C Glycine max
0.421
-
 NAD+ pH 8.0, 25°C Glycine max
0.53
-
 NAD+ pH 8.0, 25°C Glycine max
0.731
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
0.757
-
 L-homoserine cosubstrate NAD+, pH 8.0, 25°C Glycine max
2.07
-
 NADP+ pH 8.0, 25°C Glycine max
5.29
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
10.39
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
16.22
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
17.02
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
26.73
-
 L-aspartate 4-semialdehyde cosubstrate NADH, pH 8.0, 25°C Glycine max
28.54
-
 L-aspartate 4-semialdehyde cosubstrate NADPH, pH 8.0, 25°C Glycine max
61.29
-
 NADH pH 8.0, 25°C Glycine max
90.61
-
 NADH pH 8.0, 25°C Glycine max
101.4
-
 NADPH pH 8.0, 25°C Glycine max
118.9
-
 NADH pH 8.0, 25°C Glycine max
296.2
-
 NADPH pH 8.0, 25°C Glycine max
331.2
-
 NADPH pH 8.0, 25°C Glycine max