Protein Variants | Comment | Organism |
---|---|---|
additional information | design of an artificial allosteric enzyme to sense an unnatural signal for a precise and dynamical control of fluxes of growth-essential but byproduct pathways in metabolic engineering of industrial microorganisms. The natural threonine binding sites of the enzyme are engineered to a lysine binding pocket. The reengineered enzyme only responds to lysine inhibition but not to threonine | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | allosteric regulation of recombinant engineered homoserine dehydrogenase by nonnatural inhibitor L-lysine | Corynebacterium glutamicum | |
L-threonine | the natural threonine binding sites of the enzyme are predicted and verified by mutagenesis experiments | Corynebacterium glutamicum | |
additional information | the natural threonine binding sites of the enzyme are engineered to a lysine binding pocket. The reengineered enzyme only responds to lysine inhibition but not to threonine | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | P08499 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
HSD | - |
Corynebacterium glutamicum |
HSDH | - |
Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is naturally allosterically regulated by threonine and isoleucine | Corynebacterium glutamicum |