Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-hydroxybutyrate | induces the enzyme when contained in the growth medium | Acidovorax sp. | |
dithiothreitol | - |
Acidovorax sp. | |
glycerol | - |
Acidovorax sp. | |
Lactate | induces the enzyme when contained in the growth medium | Acidovorax sp. | |
succinate | induces the enzyme when contained in the growth medium | Acidovorax sp. |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21(DE3) | Acidovorax sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inhibition and destabilization of the enzyme at 10 mM | Acidovorax sp. | |
glucose | inhibits the enzyme when contained in the growth medium | Acidovorax sp. | |
Hg2+ | inhibition at 1 mM, and destabilization at 10 mM | Acidovorax sp. | |
Zn2+ | inhibition and destabilization of the enzyme at 10 mM | Acidovorax sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
NADH | reduction reaction, pH 5.0, 55°C | Acidovorax sp. | |
0.089 | - |
NAD+ | oxidation reaction, pH 8.5, 55°C | Acidovorax sp. | |
0.24 | - |
acetoacetate | reduction reaction, pH 5.0, 55°C | Acidovorax sp. | |
0.45 | - |
(R)-3-hydroxybutyrate | oxidation reaction, pH 8.5, 55°C | Acidovorax sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes the enzyme at 10 mM | Acidovorax sp. | |
Mg2+ | stabilizes the enzyme at 10 mM | Acidovorax sp. | |
Mn2+ | stabilizes the enzyme at 10 mM | Acidovorax sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, SDS-PAGE | Acidovorax sp. |
110000 | - |
gel filtration | Acidovorax sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutyrate + NAD+ | Acidovorax sp. | strain SA1 can degrade poly((R)-3-hydroxybutyrate), i.e. PHB | acetoacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax sp. | Q767A0 | - |
- |
Purification (Comment) | Organism |
---|---|
native by 3 chromatographic steps, recombinant from Escherichia coli in 2chromatographic steps | Acidovorax sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72 | - |
purified native enzyme | Acidovorax sp. |
300 | - |
purified recombinant enzyme | Acidovorax sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutyrate + NAD+ | - |
Acidovorax sp. | acetoacetate + NADH + H+ | - |
r | |
(R)-3-hydroxybutyrate + NAD+ | strain SA1 can degrade poly((R)-3-hydroxybutyrate), i.e. PHB | Acidovorax sp. | acetoacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 27000, SDS-PAGE | Acidovorax sp. |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Acidovorax sp. |
D(-)-3-hydroxybutyrate dehydrogenase | - |
Acidovorax sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Acidovorax sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | - |
Acidovorax sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | reduction reaction | Acidovorax sp. |
8.5 | - |
oxidation reaction | Acidovorax sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acidovorax sp. | |
NADH | - |
Acidovorax sp. |