General Stability | Organism |
---|---|
unstable to dilution, kept diluted at 0°C for ca. 60 min it will lose 62% of activity. This inactivation is almost completely reversed by the addition of NAD+ | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
diacetyl | pH 7.5, 25°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Geobacillus stearothermophilus |
49000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
bifunctional diacetyl reductase and (R)-2,3-butanediol dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.4 | - |
pH 7.5, 25°C | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
storage at 0°C in the presence of 20% glycerol, 0.1 mM EDTA, 5 mM 2-mercaptoethanol and 0.6 mM NAD+ in TEA buffer, pH 7.5, half-life of one month | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NADH + H+ | - |
Geobacillus stearothermophilus | (S)-acetoin + NAD+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Geobacillus stearothermophilus |