Literature summary for 1.1.1.305 extracted from

Han, S.H.; Kim, B.G.; Yoon, J.A.; Chong, Y.; Ahn, J.H.
Synthesis of flavonoid O-pentosides by Escherichia coli through engineering of nucleotide sugar pathways and glycosyltransferase (2014), Appl. Environ. Microbiol., 80, 2754-2762.

Search for more literature for 1.1.1.305

Application

Application	Comment	Organism
synthesis	engineering of Escherichia coli to ynthesize the plant-specific flavonoid O-pentosides quercetin 3-O-xyloside and quercetin 3-O-arabinoside. For UDP-xylose biosynthesis, genes UXS (UDP-xylose synthase) from Arabidopsis thaliana and ugd (UDP-glucose dehydrogenase) from E.scherichia coli, are overexpressed. The gene encoding ArnA, which competes with UXS for UDP-glucuronic acid, is deleted. For UDP-arabinose biosynthesis, UXE (UDP-xylose epimerase) is overexpressed. UDP-dependent glycosyltransferases are engineered to ensure specificity for UDP-xylose and UDP-arabinose. The srains thus obtained synthesize approximately 160 mg/liter of quercetin 3-O-xyloside and quercetin 3-O-arabinoside	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	A0A140N587	-	-

Synonyms

Synonyms	Comment	Organism
ArnA	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)