BRENDA - Enzyme Database show
show all sequences of 1.1.1.306

NAD-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from Amycolatopsis methanolica

Van Ophem, P.W.; Van Beeumen, J.; Duine, J.A.; Eur. J. Biochem. 206, 511-518 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
factor
unidentified factor that is required for maximal activity with formaldehyde as substrate, only 4% activity in its absence, oxidation of alcohols do not require the factor
Amycolatopsis methanolica
methanol
can replace an unidentified activating factor
Amycolatopsis methanolica
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
80% inhibition at 5 mM
Amycolatopsis methanolica
2-(cyclohexylamino)ethanesulfonic acid-NaOH
25 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0
Amycolatopsis methanolica
acetaldehyde
15% inhibition at 1 mM
Amycolatopsis methanolica
Cu2+
complete inhibition at 1 mM
Amycolatopsis methanolica
Glycine-NaOH
100 mM at pH 9.0: 40% of the activity compared to 0.1 M diphosphate, pH 9.0
Amycolatopsis methanolica
Hg2+
complete inhibition at 0.1 mM
Amycolatopsis methanolica
KCN
55% inhibition at 2 mM
Amycolatopsis methanolica
Na2B4O7-HCl
100 mM at pH 9.0: no activity
Amycolatopsis methanolica
Tris-HCl
50 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0
Amycolatopsis methanolica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
1-Octanol
-
Amycolatopsis methanolica
1.7
-
12-Hydroxydodecanoic acid
-
Amycolatopsis methanolica
3.4
-
1-Hexanol
-
Amycolatopsis methanolica
9.6
-
formaldehyde
-
Amycolatopsis methanolica
26.5
-
1-Pentanol
-
Amycolatopsis methanolica
84
-
1-butanol
-
Amycolatopsis methanolica
324
-
1-propanol
-
Amycolatopsis methanolica
343
-
ethanol
-
Amycolatopsis methanolica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
6 atoms Zn/enzyme molecule
Amycolatopsis methanolica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
120000
-
gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+
Amycolatopsis methanolica
-
S-formylmycothiol + NADH
-
Amycolatopsis methanolica
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Amycolatopsis methanolica
-
-
-
Purification (Commentary)
Commentary
Organism
to homogeneity
Amycolatopsis methanolica
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
induction of enzyme and factor synthesis only if cells grown in methanol-containing medium
Amycolatopsis methanolica
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.03
-
cell free extract, in the absence of an unknown activating factor
Amycolatopsis methanolica
0.08
-
cell free extract, in the presence of an unknown activating factor
Amycolatopsis methanolica
6.9
-
substrate: ethanol
Amycolatopsis methanolica
15
-
substrate: formaldehyde
Amycolatopsis methanolica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NAD+
-
288324
Amycolatopsis methanolica
n-butanal + NADH
-
288324
Amycolatopsis methanolica
?
12-hydroxydodecanoic acid + NADH
-
288324
Amycolatopsis methanolica
? + NAD+
-
288324
Amycolatopsis methanolica
?
formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+
-
288324
Amycolatopsis methanolica
S-formylmycothiol + NADH
-
288324
Amycolatopsis methanolica
-
Subunits
Subunits
Commentary
Organism
trimer
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
formaldehyde as substrate
Amycolatopsis methanolica
10.2
-
alcohols as substrates
Amycolatopsis methanolica
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Amycolatopsis methanolica
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
factor
unidentified factor that is required for maximal activity with formaldehyde as substrate, only 4% activity in its absence, oxidation of alcohols do not require the factor
Amycolatopsis methanolica
methanol
can replace an unidentified activating factor
Amycolatopsis methanolica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Amycolatopsis methanolica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
80% inhibition at 5 mM
Amycolatopsis methanolica
2-(cyclohexylamino)ethanesulfonic acid-NaOH
25 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0
Amycolatopsis methanolica
acetaldehyde
15% inhibition at 1 mM
Amycolatopsis methanolica
Cu2+
complete inhibition at 1 mM
Amycolatopsis methanolica
Glycine-NaOH
100 mM at pH 9.0: 40% of the activity compared to 0.1 M diphosphate, pH 9.0
Amycolatopsis methanolica
Hg2+
complete inhibition at 0.1 mM
Amycolatopsis methanolica
KCN
55% inhibition at 2 mM
Amycolatopsis methanolica
Na2B4O7-HCl
100 mM at pH 9.0: no activity
Amycolatopsis methanolica
Tris-HCl
50 mM at pH 9.0: 80% of the activity compared to 0.1 M diphosphate, pH 9.0
Amycolatopsis methanolica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2
-
1-Octanol
-
Amycolatopsis methanolica
1.7
-
12-Hydroxydodecanoic acid
-
Amycolatopsis methanolica
3.4
-
1-Hexanol
-
Amycolatopsis methanolica
9.6
-
formaldehyde
-
Amycolatopsis methanolica
26.5
-
1-Pentanol
-
Amycolatopsis methanolica
84
-
1-butanol
-
Amycolatopsis methanolica
324
-
1-propanol
-
Amycolatopsis methanolica
343
-
ethanol
-
Amycolatopsis methanolica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
6 atoms Zn/enzyme molecule
Amycolatopsis methanolica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
120000
-
gel filtration
Amycolatopsis methanolica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+
Amycolatopsis methanolica
-
S-formylmycothiol + NADH
-
Amycolatopsis methanolica
-
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity
Amycolatopsis methanolica
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
induction of enzyme and factor synthesis only if cells grown in methanol-containing medium
Amycolatopsis methanolica
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.03
-
cell free extract, in the absence of an unknown activating factor
Amycolatopsis methanolica
0.08
-
cell free extract, in the presence of an unknown activating factor
Amycolatopsis methanolica
6.9
-
substrate: ethanol
Amycolatopsis methanolica
15
-
substrate: formaldehyde
Amycolatopsis methanolica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NAD+
-
288324
Amycolatopsis methanolica
n-butanal + NADH
-
288324
Amycolatopsis methanolica
?
12-hydroxydodecanoic acid + NADH
-
288324
Amycolatopsis methanolica
? + NAD+
-
288324
Amycolatopsis methanolica
?
formaldehyde + 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol + NAD+
-
288324
Amycolatopsis methanolica
S-formylmycothiol + NADH
-
288324
Amycolatopsis methanolica
-
Subunits (protein specific)
Subunits
Commentary
Organism
trimer
3 * 40000, SDS-PAGE
Amycolatopsis methanolica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
formaldehyde as substrate
Amycolatopsis methanolica
10.2
-
alcohols as substrates
Amycolatopsis methanolica
Other publictions for EC 1.1.1.306
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724589
Yoshida
Gene expression analysis of me ...
Rhodococcus erythropolis, Rhodococcus erythropolis N9T-4
Biosci. Biotechnol. Biochem.
75
123-127
2011
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690518
Lee
Thiols in nitric oxide synthas ...
Nocardia sp., Nocardia sp. NRRL 5646
Appl. Environ. Microbiol.
73
3095-3097
2007
1
1
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654527
Vogt
The metabolism of nitrosothiol ...
Mycobacterium smegmatis, Mycobacterium smegmatis mc2
Biochem. J.
374
657-666
2003
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5
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1
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4
1
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1
1
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288322
Misset-Smits
Mycothiol, 1-O-(2'-[N-acwtyl-L ...
Amycolatopsis methanolica, Rhodococcus erythropolis
FEBS Lett.
409
221-222
1997
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1
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2
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5
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2
1
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2
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1
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288323
Norin
Mycothiol-dependent formaldehy ...
Amycolatopsis methanolica
Eur. J. Biochem.
248
282-289
1997
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288324
Van Ophem
NAD-linked, factor-dependent f ...
Amycolatopsis methanolica
Eur. J. Biochem.
206
511-518
1992
2
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9
8
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1
2
1
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7
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1
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1
4
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3
1
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2
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1
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9
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